Categorization of mycotoxin producing fungi in leguminous crops and its eco-friendly management under in vitro condition

DOI: 10.31830/2454-1761.2024.CR-932    | Article Id: CR-932 | Page : 79-86
Citation :- Categorization of mycotoxin producing fungi in leguminous crops and its eco-friendly management under in vitro condition. Crop Res. 59: 79-86
Address : Rajiv Gandhi South Campus, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi-221005, Uttar Pradesh, India
Submitted Date : 30-09-2023
Accepted Date : 1-01-2024


Four legume crops, viz., groundnut (Arachis hypogea L.), soybean (Glycine max (L.) Merr.) of Kharif season, chickpea (Cicer arietinum L.), and lentil (Lens esculentum L.) of Rabi season belonging to family Fabaceae were taken for investigation of seed-borne mycoflora. Since these are severely affected by many plant-pathogenic fungi at various stages of the whole life cycle, they were chosen for the study. Many post-harvest fungal pathogens cause severe loss by contaminating the grains with various mycotoxins and also deteriorate the nutritional quality of grains. The goal of any agricultural system is to increase the yield of crops as per the population demand. Various eco-friendly management practices were performed on highly mycotoxin-producing isolates of Aspergillus flavus and A. niger isolated from these grains. Four Trichoderma isolates (T2, T3, T4 and T5) were used as a biocontrol agent in the dual culture method to check antagonism between various Aspergillus isolates and Trichoderma isolates. It gave a satisfactory result in controlling mycelial growth. Achyranthes aspera leaf and stem phytoextract (10%, 20%, 30%, and 40%) was used as a botanical treatment in the well diffusion method, which successfully restricts the Aspergillus spp. mycelial growth.


Achyranthes aspera Aspergillus spp. legumes seed mycoflora Trichoderma spp. 


Alam, M. T., Karim, M. M. and Khan, S. N. (2009). Antibacterial activity of different organic extracts of Achyranthes aspera and Cassia alataJ. Sci. Res.  1: 393-98. doi:10.3329/ JSR.V1I2.2298.
Al-Ani, L. K. T. (2018). Trichoderma: beneficial role in sustainable agriculture by plant disease management. In: Plant Microbiome: Stress Response, Springer, Singapore. pp. 105-26.
Habschied, K., Krstanović, V., Zdunić, Z., Babić, J., Mastanjević, K. and Šarić, G. K. (2021). Mycotoxins biocontrol methods for healthier crops and stored products. J. Fungi 7: doi:10.3390/jof7050348.
Kubheka, B. P. and Ziena, L. W. (2022). Trichoderma: A Biofertilizer and a Bio-Fungicide for Sustainable Crop Production. In: Trichoderma-Technology and Uses. IntechOpen. doi.org/10.5772/intechopen.102405.
Masemola, B. M., Gerrano, A. S., Yobo, K. and Derera, J. (2019). Phenotypic characterization of mycotoxin resistant maize (Zea mays L.) inbred lines. Res. Crop. 20: 791-97.
Nargatti, P., Patil, S. and Wadkar, K. (2021). Phytochemical profile and pharmacological aspects of Achyranthes aspera Linn-An overview. J. Pharm. Res. Int. 33: 187-206. doi:10.9734/JPRI/2021/V33I34B31860.
O'Rourke, J. A., Bolon, Y. T., Bucciarelli, B. and Vance, C. P. (2014). Legume genomics: understanding biology through DNA and RNA sequencing. Ann. Bot. 113: 1107-20. doi: 10.1093/aob/mcu072.
Ren, X., Branà, M. T., Haidukowski, M., Gallo, A., Zhang, Q., Logrieco, A. F., Li, P., Zhao, S. and Altomare, C. (2022). Potential of Trichoderma spp. for biocontrol of aflatoxin-producing Aspergillus flavusToxins 14: doi:10.3390/toxins14020086.
Sampaio, A. M., Araújo, S. D. S., Rubiales, D., and Vaz Patto, M. C. (2020). Fusarium wilt management in legume crops. Agronomy 10: doi:10.3390/agronomy10081073.
Youssef, N. H. (2019a). Role of chitosan and some plant parts wraps as alternative interior edible coat surrounding semi-hard cheese in inhibiting fungal growth and mycotoxins migration. Res. Crop. 20: 869-79.
Youssef, N. H. (2019b). Comparison between certain chitosan wraps and cellophane wraps on fungal inhibition and mycotoxins migration. Res. Crop. 20: 105-12.
Zhou, G., Liao, B., Li, Q., Xu, J. and Chen, S. (2021). Establishing a Genomic Database for the Traditional Medicinal Plants in the Brazilian Pharmacopoeia. Chinese Med. 16:  doi:10.1186/s13020-021-00484-5.

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