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Effect of auxins and cytokinins biostimulant formulations on the growth of Citron watermelon (Citrullus lanatus var. Citroides)

Citation :- Effect of auxins and cytokinins biostimulant formulations on the growth of Citron watermelon (Citrullus lanatus var. Citroides). Res. Crop. 27: 395-403
TSEKE PONTSHO EDMUND, MAHLABANE MASHOTO AND MABOKO MPHOSI pontsho.tseke@ul.ac.za
Address : University of Limpopo, Department of Plant Production, Soil Science and, Agricultural Engineering, Private Bag X1106, Sovenga 0727, South Africa
Submitted Date : 1-04-2026
Accepted Date : 23-05-2026

Abstract

Climate change, soil degradation, and excessive reliance on synthetic fertilizers demand sustainable alternatives to improve crop productivity and resilience.
Auxin- and cytokinin-based biostimulants, such as seaweed extracts from Ecklonia maxima, offer potential benefits, yet their optimal concentrations and effects on the growth of citron watermelon remain insufficiently understood. Therefore, this study aimed to investigate the impact of different concentrations of auxin- and cytokinin-based biostimulants on the growth attributes of citron watermelon (Citrullus lanatus var. citroides), with experiments conducted and validated during the autumn season (February–April) of 2025.  The experiment was laid out in a randomized complete block design with biostimulant concentrations ranging from 0 to 60 mL per 2000 mL, replicated ten times; treatment means were compared using the Waller–Duncan multiple range test at 5% probability, and regression analysis was performed in RStudio (v2026) using a quadratic model to estimate optimum concentration. Auxins and cytokinins significantly (P ≤ 0.05) affected vine length, leaf number, chlorophyll content, stem diameter, and dry shoot mass. Vine length, number of leaves, chlorophyll content, stem diameter, and dry shoot mass all had quadratic relationships with auxins and cytokinins, with models explaining 98%, 99%, 96%, 98%, and 96% of the variation, respectively. The treatment had no significant effect (P ≥ 0.05) on root length or dry root mass, which showed quadratic correlations with auxins and cytokinins. Models explained 63% and 64% of the variance, respectively. The findings in the current study provided evidence that increasing biostimulants at low concentrations had little effect on plant growth in Citron watermelon plants.

Keywords

Auxins cytokinins healthy plants plant hormones Citron watermelon

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