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Optimising in vitro growth, chlorophyll content and secondary metabolite production of Curcuma zedoaria L. through auxin and cytokinin combinations 


Citation :- Optimising in vitro growth, chlorophyll content and secondary metabolite production of Curcuma zedoaria L. through auxin and cytokinin combinations. Res. Crop. 27: 389-394
MURGAYANTI, FARIDA, CITRA BAKTI, ALIFIYAN ANUR AND EVA APRILIA murgayanti@unpad.ac.id
Address : Department of Agronomy, Faculty of Agriculture, Universitas Padjadjaran, Jl. Raya Bandung Sumedang Km. 21, Jatinangor, Sumedang, 45363, Indonesia
Submitted Date : 23-02-2026
Accepted Date : 20-04-2026

Abstract

White turmeric is a medicinal rhizome with high pharmaceutical value due to its rich secondary metabolite content. However, conventional propagation is constrained by long growth cycles and susceptibility to diseases. In vitro tissue culture using plant growth regulators (PGRs) offers an alternative approach to improve propagation efficiency and metabolite production. This study evaluated the effects of auxin and cytokinin combinations on plantlet growth and metabolite accumulation of C. zedoaria under in vitro conditions. The experiment was arranged in a completely randomised design with 13 treatment combinations, including a control and combinations of benzyladenine, thidiazuron, or meta-topolin with naphthalene acetic acid or indole acetic acid at concentrations of 1 and 2 ppm. The combination of 1 ppm thidiazuron with 1 ppm naphthalene acetic acid significantly increased shoot multiplication (22.33 shoots per explant), while 2 ppm thidiazuron combined with 1 ppm indole acetic acid resulted in the highest flavonoid content (1.62 mg QE/100 g). These findings demonstrate that appropriate auxin–cytokinin combinations can effectively enhance in vitro propagation and metabolite production of white turmeric.

Keywords

In vitro plant growth regulator secondary metabolites tissue culture white turmeric 


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