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Research on Crops

Biostimulant-induced enhancement of germination and early seedling growth in creole and hybrid purple maize (Zea mays L.)


DOI: 10.31830/2348-7542.2025.ROC-1223    | Article Id: ROC-1223 | Page : 444-457
Citation :- Biostimulant-induced enhancement of germination and early seedling growth in creole and hybrid purple maize (Zea mays L.). Res. Crop. 26: 444-457
SANDY VILCHEZ-NAVARRO, ARTURO MORALES-PIZARRO, HENRY MOROCHO-ROMERO, SEBASTIAN CASAS-NIÑO, GABRIELA CÁRDENAS HUAMÁN, LESLIE DIANA VELARDE-APAZA AND FLAVIO LOZANO-ISLA sandygracielavilcheznavarro@gmail.com
Address : Directorate of Supervision and Monitoring of Agricultural Experimental Stations, National Institute of Agrarian Innovation, Lima, Peru
Submitted Date : 16-07-2025
Accepted Date : 3-09-2025
Online Published:

Abstract

Purple maize native to the Peruvian Andes, is a key source of anthocyanins with notable antioxidant properties. In field cultivation, seed germination and early seedling development are critical stages influencing crop establishment and yield. This study evaluated the effects of three stimulants, namely seaweed extract, sulfur dioxide, and milk whey, on the germination and early development of creole and hybrid purple maize varieties under controlled laboratory and greenhouse conditions. Seeds of each variety were subjected to seven pre-sowing treatments in a completely randomised design with three replications. Germination indices (imbibition rate, germination percentage, speed, and index) were assessed, followed by evaluation of eleven biometric traits at 15 days after emergence. Seaweed extract significantly enhanced plant height, shoot dry weight, and leaf dimensions in both varieties, particularly at 1.5 L/200 L. Sulfur dioxide improved root biomass and length, while low-dose milk whey treatments favoured moderate root growth. Creole seeds showed higher vigour in early germination, while hybrids responded better to stimulant application during early development. These findings demonstrate that biostimulants, particularly seaweed extract, can effectively enhance early growth traits in purple maize, offering potential for improving seedling establishment in sustainable production systems.

Keywords

33 33);">Biostimulants early development 33 33);"> 33 33);">germination purple maize 33 33);"> 33 33);">seaweed extract 33 33);"> 33 33);">seed vigour

References

Abdelraof, E., Nassar, I., Gomaa, I. and Aboukila, E. (2023). Valorization of cheese whey as a fertilizer: effects on maize germination and growth in clay loam and calcareous soil. Egypt. J Soil Sci. 63: 490-500.
Adekiya, A. O., Ande, O. T., Dahunsi, S. O., Ogunwole, J. and Ibaba, A. L. (2024). Impact of bio-digestate and fertilization on the soil chemical properties, growth and yield of maize (Zea mays L.). Res. Crop. 25: 33-42.
Agrodata Peru (2025). Maíz morado Perú exportación 2024 diciembre. [Online] Available at: https://agrodataperu.com/2025/02/maiz-morado-peru-exportacion-2024-diciembre.html (Accessed on 9 August 2025).
Akay, A. and Sert D. (2020). The effects of whey application on the soil biological properties and plant growth. Eurasian J. Soil Sci. 9: 349-54.
Ali, O., Ramsubhag, A. and Jayaraman, J. (2021). Biostimulant properties of seaweed extracts in plants: Implications towards sustainable crop production. Plants 10: 5-19.
Bhoopesh, G., Baradhan, G., Suresh Kumar, S. M., Kathirvelu, C. and Ramesh, S. (2024). Synergistic impact of nano-urea and microbial inoculants with varied nitrogen regimes on the yield and yield attributes of hybrid maize (Zea mays L.). Crop Res. 59: 122-28.
Chachar, Z., Lai, R., Ahmed, N., Lingling, M., Chachar, S., Paker, N. P. and Qi, Y. (2024). Cloned genes and genetic regulation of anthocyanin biosynthesis in maize, a comparative review. Front. Plant Sci. 15: 5-14.
da Silva, E. E., Baio, F. H. R., Kolling, D. F., Júnior, R. S., Zanin, A. R. A., Neves, D. C., Fontoura, J. V. P. F. and Teodoro, P. E. (2021). Variable-rate in corn sowing for maximizing grain yield. Sci. Rep. 11: 1-7.
Duangpapeng, P., Lertrat, K., Lomthaisong, K., Paul, S. M. and Suriharn, B. (2019). Variability in anthocyanins, phenolic compounds and antioxidant capacity in the tassels of collected waxy corn germplasm. Agronomy 9: 4-10.
Erenstein, O., Jaleta, M., Sonder, K., Mottaleb, K. and Prasanna, B. M. (2022). Global maize production, consumption and trade: Trends and R&D implications. Food Secur. 14: 1309-13.
Ertani, A., Francioso, O., Tinti, A., Schiavon, M., Pizzeghello, D. and Nardi, S. (2018). Evaluation of seaweed extracts from Laminaria and Ascophyllum nodosum spp. as biostimulants in Zea mays L. using a combination of chemical, biochemical and morphological approaches. Front. Plant Sci. 9: 9-11.
Escobar-Álvarez, J. L., Ramírez-Reynoso, O., Saguilán, P. C., Gutiérrez-Dorado, R., Maldonado-Peralta, M. de Los, Á. and Valenzuela-Lagarda, J. L. (2021). Viabilidad y germinación en semillas de maíz criollo del estado de Guerrero. Ecosist. Recur. Agropecu. 8: 1-7.
Fatriana-Caronge, M. W., Djawad, Y. A., Bourgougnon, N., Makkulawu, A. T. and Jumadi, O. (2020). Effect of application of algae sargassum sp. Extract to corn plants (Zea mays L.) and microbial response. IOP Conf. Ser. Earth Environ. Sci. 484: 1-6.
Gandhi, G., Gopalakrishnan, V. A. K., Veeragurunathan, V. and Ghosh, A. (2024). Unlocking the potential of tropical red and brown seaweed-based biostimulants—A comparative assessment for sustainable maize (Zea mays) production. J. Appl. Phycol. 36: 1513-31.
Guo, Z., Zhao, J., Wang, M., Song, S. and Xia, Z. (2021). Sulfur dioxide promotes seed germination by modulating reactive oxygen species production in maize. Plant Sci. 312: doi:10.1016/j.plantsci.2021.111027.
Gutiérrez-Gutiérrez, O. G., Rivero-Hernández, O., Vega-Mares, J. H., Melgoza-Castillo, A. (2022). Germination patterns on grasses present at the Chihuahuan Desert. Bot. Sci. 100: 993-96.
Han, Z., Wang, B., Tian, L., Wang, S., Zhang, J., Guo, S., Zhang, H., Xu, L. and Chen, Y. (2020). Comprehensive dynamic transcriptome analysis at two seed germination stages in maize (Zea mays L.). Physiol. Plant. 168: 205-17.
Hernández-Herrera, R. M., González-González, M. F., Velasco-Ramírez, A. P., Velasco-Ramírez, S. F., Santacruz-Ruvalcaba, F. and Zamora-Natera, J. F. (2023). Seaweed extract components are correlated with the seed germination and growth of tomato seedlings. Seeds 2: 439-45.
Huang, Y., Zhang, Y., Gao, C., Li, Z., Guan, Y., Hu, W. and Hu, J. (2018). The interactions of plant growth regulators and H2O2 during germination improvement of sweet corn seed through spermidine application. Plant Growth Regul. 85: 15–26. doi:10.1007/s10725-018-0370-z.
Husson, F., Josse, J., Le, S. and Mazet, J. (2025). FactoMineR: Multivariate exploratory data analysis and data mining (Versión 2.12) [Software]. https://cran.r-project.org/web/packages/ FactoMineR/index.html.
Jhanji, S., Goyal, E., Chumber, M. and Kaur, G. (2024). Exploring fine tuning between phytohormones and ROS signaling cascade in regulation of seed dormancy, germination and seedling development. Plant Physiol. Biochem. 207: doi:10.1016/j.plaphy.2024.108352.
Kaushal, M., Sharma, R., Vaidya, D., Gupta, A., Saini, H. K., Anand, A., Thakur, C., Verma, A., Thakur, M. and Priyanka, K. C. D. (2023). Maize: An underexploited golden cereal crop. Cereal Res. Commun. 51: 3-14.
Lenth, R. V., Bolker, B., Buerkner, P., Giné-Vázquez, I., Herve, M., Jung, M., Love, J., Miguez, F., Piaskowski, J., Riebl, H. and Singmann, H. (2024). emmeans: Estimated Marginal Means, aka Least-Squares Means (Version 1.10.4) [Software]. Available at: https://cran.r-project.org/web/packages/emmeans/index.html.
Lozano-Isla, F., Benites-Alfaro, O. E. and Pompelli, M. F. (2019). GerminaR: An R package for germination analysis with the interactive web application “GerminaQuant for R”. Ecol. Res. 34: 339-46.
Mamede, M., Cotas, J., Pereira, L. and Bahcevandziev, K. (2024). Seaweed polysaccharides as potential biostimulants in turnip greens production. Horticulturae 10: 18-21.
Medina-Hoyos, A., Narro-León, L. and Chávez-Cabrera, A. (2020). Cultivo de maíz morado (Zea mays L.) en zona altoandina de Perú: Adaptación e identificación de cultivares de alto rendimiento y contenido de antocianina. Sci. Agropecuaria 11: 294-98.
Narayan, O. P., Kumar, P., Yadav, B., Dua, M. and Johri A. K. (2023). Sulfur nutrition and its role in plant growth and development. Plant Signal. Behav. 18: 1-8.
Panda, S., Borkataki, S., Reddy, M. D. and Nanda, S. P. (2020). Domestication and evolution of maize. Int. J. Chem. Stud. 8: 1834-38.
Pizarro, A. M., Chero, A. A. R., Córdova, A. C. Z., Guevara, E. G., López, M. R. and Peña-Castillo, R. (2023). Efecto de diferentes dosis de ácido giberélico en la germinación de papaya (Carica papaya L.) variedad Criolla. Chil. J. Agric. Anim. Sci. 39: 394-400.
Pompelli, M. F., Jarma-Orozco, A. and Rodriguez-Páez, L. A. (2023). Imbibition and germination of seeds with economic and ecological interest: physical and biochemical factors involved. Sustainability 15: 3-10.                                                                                                            
Pourbabaee, A. A., Dinekaboodi, S. K., Hosseini H. S. D., Alikhani, H. A. and Emami, S. (2020). Potential application of selected sulfur-oxidizing bacteria and different sources of sulfur in plant growth promotion under different moisture conditions. Commun. Soil Sci. Plant Anal. 51: 735-45. doi:10.1080/00103624.2020.1729377.
R Core Team (2024). R: The R Project for Statistical Computing (Version 4.4.1) [Software]. R Foundation for Statistical Computing. Available at: https://www.r-project.
Rabanal-Atalaya, M. and Medina-Hoyos, A. (2021). Análisis de antocianinas en el maíz morado (Zea mays L.) del Perú y sus propiedades antioxidantes. Rev. Terra Latinoam. 39: 5-10.
Riffat, A. and Ahmad, M. S. A. (2020). Alleviation of adverse effects of salt stress on growth of maize (Zea mays L.) by sulfur supplementation. Pak. J. Bot. 52: 765-72.
Ruesta-López, M., Zurita-Chinguel, L., Lizano-Pintado, M., Delgado-Vite, M., Zapata-Durand, D., Jiménez-Castillo, J., Peña-Castillo, R., Galecio-Julca, M., Chanduví-García, R. and Pizarro, D. A. M. (2024). Biostimulant and imbibition times on passion fruit (Passiflora edulis S., Passifloraceae) seed germination. Trop. Subtrop. Agroecosyst. 27: 3-10.
Sairam, M., Maitra, S., Ray, S., Maity, B. and Pradhan, P. (2025). Influence of need-based nutrient management on yield and nutrient use efficiency of Rabi maize (Zea mays L.) under sandy loam soils of Odisha, India. Farm. Manage. 10: 11-19.
Scott, S. J., Jones, R. A. and Williams, W. A. (1984). Review of data analysis methods for seed germination. Crop Sci. 24: 1192-99.
Shukla, P. S. and Prithiviraj, B. (2021). Ascophyllum nodosum biostimulant improves the growth of Zea mays grown under phosphorus impoverished conditions. Front. Plant Sci. 11: 2-4.
Sousa, M. J. and Malcata, F. X. (1998). Identification of peptides from ovine milk cheese manufactured with animal rennet or extracts of Cynara cardunculus as coagulant. J. Agric. Food Chem. 46: 4034-41.
Torre, A. B., Pérez-Ramírez, E., Morales-García, Y. E., Muñoz-Rojas, J., Díaz-Ruíz, R. and Morales-Almora P. (2023). Efecto del uso de lactosuero dulce en el riego de alfalfa y maíz. Rev. Mex. Cienc. Agríc. 29: 2-8.
Vieira, H., Martins, J. V. da S., Barreto, G. G., Gomes, R. dos S. S., Silva, E. C. and Nascimento L. C. (2019). Sanitary and physiological quality of ‘purple’ corn (Zea mays L.) seeds submitted to thermotherapy. Arq. Inst. Biol. 86: 2-6.
Zenda, T., Liu, S., Dong, A. and Duan H. (2021). Revisiting sulphur - the once neglected nutrient: it’s roles in plant growth, metabolism, stress tolerance and crop production. Agriculture 11: 11-19.


 
 

 
 

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