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Methods of biotechnology in the improvement of promising potato hybrids (Solanum tuberosum L.)

DOI: 10.31830/2348-7542.2021.023    | Article Id: 023 | Page : 96-99
Citation :- Methods of biotechnology in the improvement of promising potato hybrids (Solanum tuberosum L.). Res. Crop. 22: 96-99
I. V. Kim, A. S. Chibizova, E. V. Shischenko, P. V. Fisenko, T. N. Chekushkina, E. N. Barsukova, D. I. Volkov, A. G. Klykov 80@mail.ru
Address : Federal State Scientific Institution “Federal Scientific Center of Agrobiotechnology in the Far East named after A. K. Chaika”: 30, Volozhenina Street, Stl. Timiryazvsky, Ussuryisk, Primorskykrai, 692539, Russia

Abstract

The spread of viruses through seed tubers from generation to generation is a major limitation of potato yield. Having virus-free material available is extremely important. In this regard, the effectiveness of various antiviral drugs in chemotherapy in improving the health of promising potato hybrids for reproduction in the in vitro collection was analyzed. The viral load in hybrid lines was determined using ELISA and PCR methods. It was found that using ribavirin and chitosan during the 25-day passaging of potato microclones does not completely eliminate viral infection. However, the samples with the lowest concentration of pathogens that will be used in further rehabilitation were identified.

Keywords

Microplants in vitro  Micropropagation  Potato  Seed production  Solanum tuberosum L.  Variety  Virus.

References

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