Seedling nature and enzyme action liable to enhancement of seedling vigour are crucial to ensure the seed quality. Recently, the beneficial effect of seed invigoration treatment with organics has been well established to maintain vigour and viability of seed. The experiment considered two years (2013–14 and 2014–15) observations where the extracted solutions of cow dung and cow urine were used as seed treatments (symbolized as T0 to T9) in alone or in combination considering its various concentrations with control (water). The stored seed (six months) under ambient condition was capable in leading through application of T9 (2% cow dung solution+75% cow urine) followed by T5 (2% cow dung solution) where the treatments like T4 (1% cow dung solution), T8 (1% cow dung solution+100% cow urine) quantified greater enactment in a few cases. The treatments showed significant influence for various seedling parameters like speed of germination, dry weight and vigour index in two succeeding years. There was a nonsignificant variation in year and interaction of treatment-year combinations. Normally, the enzyme alpha amylase was enhanced to motivate the progression of germination. The treatment effect sustained a variable action for the enzyme where T9 and T5 were greatest. Therefore, the advantageous nature of cow dung in definite combinations viz., T5, T9 or more specifically T9 may be considered as technical tool for seed or crop production of cowpea.