This study was conducted to determine growth characteristics and total phenol, total flavonoid and DPPH radical scavenging activity of five species of the genus Artemisia under different transplanting methods. The order of shoot fresh weight in five species of the genus Artemisia among different transplanting methods was roots with 1 cm stem transplanting = root transplanting > whole plant transplanting at 90 days after transplanting (DAT) or cutting (DAC). The shoot fresh weight at 90 DAT was highest in A. princeps Pamp., whereas shoot fresh weight of regrowth at 90 DAC was highest in A. princeps Pamp. cv. Sajuarissuk. The shoot fresh weight of A. princeps Pamp. cv. Sajuarissuk was two times higher at 90 DAC than at 90 DAT. There were no differences in total phenol and total flavonoid contents or DPPH radical scavenging activities of the five species at 90 DAT or DAC among transplanting methods. However, the total phenol and total flavonoid contents and DPPH radical scavenging activities at 90 DAT were higher than at 90 DAC. In addition, the total phenol and total flavonoid contents and DPPH radical scavenging activities of A. princeps Pamp. cv. Sajabalssuk were significantly higher than those of the other four Artemisia species at 90 DAT or DAC. However, yield in Sajabalssuk was lower than that of the other four Artemisia species. Thus, Sajuarissuk can be recommended for cultivation because it showed high yield as well as relatively high antioxidant activity.