Field experiment was conducted at Instructional Farm of Rajasthan College of Agriculture, Udaipur during kharif 2015 to assess the response of soybean [Glycine max (L.) Merrill] to microbial inoculation and sulphur. The experiment was laid out in factorial randomized block design with three replications and consisted treatments viz., microbial inoculation (Control, Rhizobium, phosphate solubilizing bacteria, Rhizobium+phosphate solubilizing bacteria) and four levels of sulphur (0, 20, 40 and 60 kg S/ha). The results revealed that microbial inoculation and sulphur significantly increased the nutrient content and uptake in N, P and K and quality viz., oil content and yield, protein content and yield over control.