A field experiment was conducted at the Agronomy Instructional Farm, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar (Gujarat) during the rabi season of the years 2013–14 and 2014–15. The experiment consisted of 14 treatment combinations comprising two levels of nitrogen and seven split applications of nitrogen, and conducted in RBD with factorial concept with three replications. The soil of the experimental field was loamy sand in texture, very low in organic carbon and available nitrogen, medium in available phosphorus and potash. Significantly higher grain yield (4572 kg/ha) was recorded when crop was fertilized with 160 kg N/ha. Application of 160 kg N/ha recorded higher value in respect to nitrogen content in grain (2.021%) and in respect to only uptake of nitrogen (92.40 kg/ha), phosphorus (18.97 kg/ha) and potassium (69.89 kg/ha) by grain only nitrogen uptake by straw (28.42 kg/ha). Grain yield (4964 kg/ha) and straw yield (5792 kg/ha) was significantly higher when nitrogen was applied as 50% at sowing, 25% at CRI stage and 25% at 1st node stage. Nitrogen content in wheat grain (1.953%) and straw (0.550%) was significantly higher with treatment S7. Nitrogen uptake by grain (95.54 kg/ha), phosphorus uptake by grain (20.92 kg/ha) and straw (1.334 kg/ha) and potassium uptake by grain (75.97 kg/ha) and straw (24.90 kg/ha) were significantly higher when nitrogen was applied as 50% at sowing, 25% at CRI stage and 25% at 1st node stage. In case of nitrogen uptake by straw yield (30.90 kg/ha), it was higher under treatment S5. Significantly, the highest available nitrogen in soil after harvest of crop was recorded in treatment S7.