Abstract

The present investigation was carried out to develop an efficient in vitro protocol for production of healthy quality planting material of strawberry. Nodal segment of strawberry gave rise to multiple shoots when cultured on MS medium supplemented with different concentration of kinetin and BAP with IAA and NAA. The highest response of shoot multiplication was obtained in MS medium containing 2.5 mg/l kinetin+0.1 mg/l IAA. The regenerated shoots were rooted in half strength MS medium with IBA, IAA, NAA and control (without growth regulators). The highest number of roots was produced in half strength MS medium 1.0 mg/l NAA, however, maximum root length and rooting percentage were obtained in medium supplemented with 1.0 mg/l IBA. The plantlets, thus developed, were hardened and successfully established in mixture of coco peat, vermiculite and perlite in ratio of 3: 1: 1. The plants raised through this technique exhibited normal growth and fruit setting.