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In vitro compatibility of entomopathogenic fungi, Beauveria bassiana (Bals.-Criv.) Vuill. with leaf extracts of Chromolaena odorata (Asteraceae)

DOI: 10.5958/2348-7542.2016.00028.0    | Article Id: 028 | Page : 157-162
Citation :- In vitro compatibility of entomopathogenic fungi, Beauveria bassiana (Bals.-Criv.) Vuill. with leaf extracts of Chromolaena odorata (Asteraceae). Res. Crop. 17: 157-162
S. Kalita, Purnima Das, L. K. Hazarika, P. Dutta, Gautam Handique, Karishma Das handique.gautam@yahoo.com
Address : Department of Entomology, Assam Agricultural University, Jorhat-785 013, Assam, India

Abstract

The compatibility of Beauveria bassiana (Bals.-Criv.) Vuill. with Chromolaena odorata (L.) King & H. Rob. (Asteraceae) was evaluated in vitro. Aqueous and solvent extracts of leaves of C. odorata at concentrations of 0.1, 0.5 and 1% were evaluated on the growth, conidial density and germination of B. bassiana by using the poisoned food technique. The extracts exhibited varying effects on growth of the fungus ranging from toxicity to inhibitory action, which increased with increase in the concentrations. The reduction percentage of radial growth of B. bassiana over control was found to be significantly less with the aqueous extracts of C. odorata. Inhibitory action was found to be highest (96.93%) with the chloroform extracts at 1% concentration. Similarly, as the concentrations increased from 0.1 to 1%, there was a significant reduction in the conidial density and germination in all the test concentrations. Based on the findings, the order of compatibility based on percentage reduction of radial growth is as follows: water > petroleum ether > methanol > acetone > chloroform. Hence, the aqueous extracts of C. odorata at 0.1% concentration can be integrated along with B. bassiana in any biointensive pest management programme.

Keywords

Beauveria bassiana  biopesticide  Chromolaena odorata  compatibility  poisoned food technique  T-value.

References

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