Fenugreek is of utmost importance due to its high nutritive value, medicinal properties and industrial uses. The present investigation aims at the estimation of some micronutrients, namely, iron, zinc, manganese and copper from seeds of fenugreek genotypes using atomic absorption spectrophotometer. Twenty-four genotypes of fenugreek along with two standard checks, namely, Pant Ragini and Pusa Early Bunchingwere collected from different parts of India andmicronutrient contents of each genotype were determined at G. B. P. U. A. & T. Laboratory, Pantnagar. PFG-20 was richest in iron and manganese content among all genotypes. The lowest zinc content was found in Pant Ragini, while the maximum zinc content was found in PFG-8. Highest content of copper was found in PFG-2 (1.23±0.017 mg/100 g) followed by PFG-4 (1.11±0.015 mg/100 g), whereas minimum copper content was found in PFG-9 (0.58±0.008 mg/100 g).