Field experiments were conducted to study the impact of stabilized N fertilizer on yield, nutrient uptake and soil quality under maize-wheat cropping sequence at Fertilizer Research Farm, Uttaripura, Kanpur Nagar (U. P.), Chandra Shekhar Azad University of Agriculture & Technology, Kanpur during 2010–11 and 2011–12. Results indicated that grain and straw yield of maize-wheat significantly increased with the use of different urea A Lab over (T1) urea lab 8041 (plain urea). Significant increase in yield of maize-wheat was also noted within each urea A Lab up to urea A Lab 8044. The highest grain yield of maize and wheat was noted 33.15 and 56.75 q/ha, respectively, with urea A Lab 8045 (T6). The per cent response in grain of maize-wheat at T6 over T2 was noted (6.75 and 10.25 q/ha) with corresponding per cent response 25.56 and 22.04, respectively. Nutrient uptake (NPK) also significantly increased with various urea A Lab over urea Lab (T2). Protein content in both maize and wheat positively responded with the use of different urea A Lab over urea Lab. The response of various urea A Lab 100% was almost at par with 125% urea Lab 8046 (plain urea).