This study was conducted at Department of Zoology, University of Gujrat, Pakistan during 2016–17 as a term paper for Master of Philosophy. The data regarding the role of enzymes in improving the soil nutrition were obtained and compiled through a thorough review of various published research articles of international reputed journals and relevant books. Enzymes are the vital activators in life processes, likewise in the soil they are known to play a substantial role in maintaining soil health and its environment. The enzymatic activity in the soil is mainly of microbial origin, being derived from intracellular, cell-associated or free enzymes. A unique balance of chemical, physical and biological (including microbial especially enzyme activities) components contribute to maintaining soil health. Healthy soils are essential for the integrity of terrestrial ecosystems to remain intact or to recover from disturbances, such as drought, climate change, pest infestation, pollution, and human exploitation including agriculture.