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Comparative GC-MS analysis of bioactive phytocompounds in the aqueous and hydro-ethanolic extracts from rhizomes of Nymphoides indica L.


Citation :- Comparative GC-MS analysis of bioactive phytocompounds in the aqueous and hydro-ethanolic extracts from rhizomes of Nymphoides indica L.. Crop Res. 61: 180-186
BANAJA PANIGRAHI, DINKAR GAIKWAD, ASHIRBAD NANDA AND SATYABRATA NANDA sbn.satyananda@gmail.com
Address : School of Biotechnology, Centurion University of Technology and Management, Bhubaneswar, Odisha-752050, India
Submitted Date : 15-11-2025
Accepted Date : 5-01-2026

Abstract

Identification of phytocompounds in plants is crucial to recognise their potential therapeutic uses, yet extraction outcomes and metabolite profiles vary widely with solvent and method. Although Nymphoides indica is traditionally valued for its medicinal properties, scientific information on its detailed bioactive composition remains limited. Therefore, it is essential to compare the GC-MS profiles of its aqueous and hydroethanolic rhizome extracts to identify key metabolites and support its pharmacological validation. This study aimed to identify the bioactive phytochemicals in aqueous and hydro-ethanoic extracts of rhizomes of N. indica, a common hydrophyte, by gas chromatography-mass spectrometry (GC-MS) analysis. The air-dried rhizomes of N. indica were coarsely powdered and subsequently extracted with distilled water and 70% ethanol as solvents. In total, two extracts have been obtained in the preparation of plant extracts, including an aqueous (ARNI) and a hydroethanolic extract (HRNI) of N. indica rhizome. The extracts were subsequently analysed by GC-MS, which detected 13 different bioactive compounds with varying molecular weights, 6 compounds in ARNI, and 11 compounds in HRNI. Compounds identified from these extracts showed high biological relevance and pharmacological properties. This study gives a comprehensive knowledge of the detection and identification of miscellaneous bioactive phytochemicals from the rhizome of N. indica. This provides a basis for the biological and biochemical development of rapidly discovered biologically and pharmacologically bioactive phytochemicals of this plant.

Keywords

Bioactive compounds bioprospecting GC-MS analysis Nymphoides indica L. phytocompounds

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