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In vitro regeneration and phytochemical profiling of Ageratum conyzoides L.  


Citation :- In vitro regeneration and phytochemical profiling of Ageratum conyzoides L.. Crop Res. 61: 195-201
NIDHI SELWAL, VIJAY KUMAR AND SARVJEET KUKREJA sarvjeet.24849@lpu.co.in; nidhiselwal947.ns@gmail.com
Address : School of Bioengineering and Biosciences, Lovely Professional University, Phagwara-144411, Punjab, India
Submitted Date : 28-10-2025
Accepted Date : 15-01-2026

Abstract

Ageratum conyzoides L., long used in ethnomedicine for its antioxidant, anti-inflammatory, anticancer, antidiabetic and allelopathic effects, is one such species. To support its sustainable utilization, this study, conducted from March–December 2024, hypothesized that optimizing the balance of cytokinins and auxins can enhance shoot proliferation and secondary metabolite accumulation in Ageratum conyzoides L. Nodal explants were cultured on Murashige and Skoog (MS) medium supplemented with various plant growth regulators. Shoot proliferation was highest on media supplemented with 1.0 mg/L benzyladenine (BA), while rooting was most effective with 0.5 mg/L naphthaleneacetic acid (NAA); higher auxin concentrations inhibited root development. Extracts from in vitro grown aerial parts using multiple solvents were analyzed by GC-MS, identifying major compounds including Phenol, 2,4-bis (1,1-dimethylethyl)-, phosphite (3:1) (≈ 27.7 %), Neophytadiene (≈ 17.4 %), a benzopyran derivative (6,7-dimethoxy-2,2-dimethyl) (≈ 11.2 %), (E)-β-Farnesene (≈ 10.1 %), and Caryophyllene (≈ 6.5 %). These findings demonstrate that tissue culture provides a reliable propagation route while conserving the phytochemical integrity of A. conyzoides, supporting ex-situ conservation and sustainable utilization.

Keywords

A. conyzoides GC-MS analysis in vitro regeneration phytochemical profiling shoot proliferation 


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