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Enhancing soybean (Glycine max M.) productivity through synergistic application of hormones and nutrients synergy


DOI: 10.31830/2454-1761.2026.CR-1083    | Article Id: CR-1083 | Page : 87-95
Citation :- Enhancing soybean (Glycine max M.) productivity through synergistic application of hormones and nutrients synergy. Crop Res. 61: 87-95
NAGAJOTHI RAJASEKARAN, V SUGANYA, VINCENT S., JEYAKUMAR P. AND S. JIDHU VAISHNAVI sjvaishnavi@gmail.com
Address : Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore-641003, Tamil Nadu, India
Submitted Date : 28-10-2025
Accepted Date : 13-01-2026

Abstract

Soybean is a vital global legume crop, yet its productivity is often constrained by nutrient limitations, suboptimal physiological efficiency, and limited access to growth-enhancing technologies. Although plant growth regulators (PGRs) and nutrients individually improve growth, their combined effects on soybean physiology and yield remain poorly understood. Hence, there is a critical need to evaluate integrated PGR–nutrient strategies to enhance soybean productivity and sustainability under field conditions. This study evaluated the synergistic effects of foliar-applied plant growth regulators (PGRs) viz., Gibberellic acid (GA₃) and melatonin, and/or combined with a micronutrient-enriched spray (DAP, MnSO₄, FeSO₄, and ammonium molybdate) on soybean performance under field conditions in Tamil Nadu Agricultural University, Coimbatore during Kharif season of 2023. Six foliar treatments including control, nutrients, GA₃, nutrients with GA₃, melatonin, and nutrients with melatonin were applied at 30 and 40 DAS and evaluated in a randomized block design to assess growth, photosynthetic efficiency, biochemical traits, and yield components. The combined application of melatonin and nutrients (T6) significantly enhanced plant height, leaf area, chlorophyll content, nitrate reductase activity, and seed yield per plant (19.6 g), outperforming the other treatments. Melatonin alone (T5) yielded the highest seed protein content (37.58 mg/g), thereby highlighting its role in nutritional enhancement. The results demonstrate that integrating PGRs with nutrient optimize soybean metabolism, reproductive success, and seed quality offering a scalable strategy for sustainable legume intensification.

Keywords

Gibberellins melatonin nutrient mixture physiology soybean

References

Ali, K. A., Noraldeen, S. S.  and Yaseen, A. A. (2021). An evaluation study for chlorophyll estimation techniques. Sarhad J. Agric. 37: 1458-65.
Amoanimaa-Dede, H., C. Su, C., Yeboah, A., Zhou, H., Zheng, D. and Zhu, H. (2022). Growth regulators promote soybean productivity: A review. Peer J. 10: doi:10.7717/peerj.12556.
Band, L. R., Úbeda-Tomás, S., Dyson, R. J., Middleton, A. M., Hodgman, T. C., Owen, M. R., Jensen, O. E., Bennett, M. J. and King, J. R. (2012). Growth-induced hormone dilution can explain the dynamics of plant root cell elongation. Proc. Natl. Acad. Sci. 109: 7577–82.
Bons, H. K. and Kaur, M. (2020). Role of plant growth regulators in improving fruit set, quality and yield of fruit crops: a review. J. Hortic. Sci. Biotechnol. 95: 137-46.                             
Danilova, E. D., Efimova, M. V., Kolomeichuk, L. V. and Kuznetsov, V. V. (2020). Melatonin supports photochemical activity of assimilation apparatus and delays senescence of leaves of monocotyledonous plants. Dokl. Biochem. Biophys. 495: 271–75.                                
Dass, A., Rajanna, G. A., Babu, S., Lal, S. K., Choudhary, A. K., et al. and Kumar, B. (2022). Foliar Application of Macro-and Micronutrients Improves the Productivity, Economic Returns, and Resource-Use Efficiency of Soybean in a Semiarid Climate. Sustain. 14: doi:10.3390/su14105825.
Duan, Y., Wang, X., Jiao, Y., Liu, Y., Li, Y., et al. and Wang, S. (2024). Elucidating the role of exogenous melatonin in mitigating alkaline stress in soybeans across different growth stages: a transcriptomic and metabolomic approach. BMC Plant Biol. 24: doi:10.1186/s12870-024-05101-9.
DuBois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. (1956). Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–56. doi:10.1021/ac60111a017.
Gong, X.-W., Liu, C.-J., Feng, N.-J., Zheng, D.-F. and Wang, C. (2017). Effects of plant growth regulators S 3307 and DTA-6 on photosynthetic characteristics and yield in soybean canopy. Plant Physiol. J. 53: 1867-76.
Jaybhaye, S. G., Deshmukh, A. S., Chavhan, R. L., Patade, V. Y. and Hinge, V. R. (2024). GA3 and BAP phytohormone seed priming enhances germination and PEG induced drought stress tolerance in soybean by triggering the expression of osmolytes, antioxidant enzymes and related genes at the early seedling growth stages. Environ. Exp. Bot. 226: doi:10.1016/j.envexpbot.2024.105870.
Kim, J. Y. and Seo, H. S. (2018). In vitro nitrate reductase activity assay from arabidopsis crude extracts. Bio-protocol 8: doi:10.21769/BioProtoc.2785.
Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall R. J. (1951). Protein measurement with the folin phenol reagent. J. Biol. Chem. 193: 265-75.         
Manu, S. M., Halagalimath, S. P., Chandranath, H. T. and Biradar, B. D. (2021). Effect of plant-growth regulators and nutrient levels on productivity and nutrient uptake of soybean (Glycine max). Indian J. Agron. 66: 108 – 11.
Mehta, S., Jain, M. P. and Singh, S. (2015). Effect of nitrogen and plant growth regulators on yield and yield attributes of soybean [Glycine max (L.) Merrill] under late sown conditions. Ann. Agri Bio Res. 20: 167-70.
Pearce, R. B., Carlson, G. E., Barnes, D. K., Hart, R. H. and Hanson, C. H. (1969). Specific leaf weight and photosynthesis in alfalfa. Crop Sci. 9: 423-26.
Prusty, A. A., Behura, A. K., Prusti, A. M., Mohapatra, A. K. B., Panda, P. K., Mishra, I. O. P., Panigrahi, R. K. and Das, T. R. (2022). Effect of foliar application of nutrients and plant growth regulators on yield and yield attributing characters of greengram (Vigna radiata L.). 40: 1229–35.
Rogach, V. V, Kuryata, V. G., Kosakivska, I. V, Voitenko, L. V, Shcherbatyuk, M. M. and Rogach, T. I. (2022). Morphogenesis, pigment content, phytohormones and yield of tomatoes under the action of gibberellin and tebuconazole. Biosyst. Divers. 30: 150-56.
Sabagh, A. E. Mbarki, L. S., Hossain, A., Iqbal, M. A., Islam, M. S., et al. (35 authors) and Muhammad, F. (2021). Potential role of plant growth regulators in administering crucial processes against abiotic stresses. Front. Agron. 3: doi:10.3389/fagro.2021.648694.
Shehzad, M., Muhammad, S. M., Muhammad, N.,  Tahir, M., Maqbool, M., Nawaz, M. A., Javeed, H. M. R. and Al-Ghamdi, A. A. (2024). Evaluating the impact of phyto-hormones on the morpho-biochemical traits of soybean through seed treatment and foliar application. J. King Saud Univ. Sci. 36: doi:10.1016/j.jksus.2024.103446.
Tu, B., Liu, C., Tian, B., Zhang, Q., Liu, X. and Herbert, S. J. (2017). Reduced abscisic acid content is responsible for enhanced sucrose accumulation by potassium nutrition in vegetable soybean seeds. J. Plant Res. 130: 551-58.
Voora, V., Bermúdez, S., Larrea, C. and Luna, E. (2024). Global market report: soybean prices and sustainability. Sustain. Commod. Marketpl. Ser-37. https://www.iisd.org/system/files/ 2024-02/2024-global-market-report-soybean.pdf.
Wang, X., Zhang, Y., Zhang, J., Li, X., Jiang, Z. and Dong, S. (2025). Effects of DA-6 and MC on the growth, physiology, and yield characteristics of soybean. BMC Plant Biol. 25: doi:10.1186/s12870-025-06310-6.
Watson. (1947). Comparative physiological studies in the growth of field crops. I. Variation in net assimilation rate and leaf area between species and varieties, and within and between years. Ann. Bot. 11: 41–76.
Wavhale, M. and Salve, U. (2024). Effect of plant growth regulators on growth and yield of soybean. A. Q. J. Life Sci. 21: 83–84.  doi:10.5958/0976-4755.2024. 00028.2.
Wilson, J. W. (1966). Effect of temperature on net assimilation rate. Ann. Bot. 30: 753 – 61.
Zhang, N., Xie, Y. D., Guo, H. J., Zhao, L. S., Xiong, H. C., et al. and Liu, L. X. (2016). Gibberellins regulate the stem elongation rate without affecting the mature plant height of a quick development mutant of winter wheat (Triticum aestivum L.). Plant Physiol. Biochem. 107: 228–36.
 
 
 
 
 
 

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