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Storage of biofertilizers containing potassium-solubilising purple nonsulfur bacteria, Cereibacter sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11 and Rhodopseudomonas palustris M-So-14


Citation :- Storage of biofertilizers containing potassium-solubilising purple nonsulfur bacteria, Cereibacter sphaeroides M-Sl-09, Rhodopseudomonas thermotolerans M-So-11 and Rhodopseudomonas palustris M-So-14. Crop Res. 60: 381-391
NGUYEN QUOC KHUONG, TRAN TRONG KHOI NGUYEN, VO YEN NGOC, NGUYEN DUC TRONG, LE THANH QUANG, LE THI MY THU AND LA CAO THANG nqkhuong@ctu.edu.vn
Address : Faculty of Crop Science, College of Agriculture, Can Tho University, Vietnam
Submitted Date : 1-09-2025
Accepted Date : 8-10-2025

Abstract

Rapid population growth, environmental pollution, and climate change are intensifying pressure on agriculture, demanding sustainable nutrient management to ensure food security. Replacing part of chemical fertilisers with bio-organic fertilisers from agricultural by-products, enriched with beneficial microbes like purple nonsulfur bacteria (PNSB), can enhance soil quality and crop yield. Storing biofertilizers in a mixture of carriers and substrates helps maintain microbial density stability for a longer period. This study was conducted to determine the optimal moisture content for storing biofertilizers with the highest viable cell density and assess the ability of biofertilizers to retain macronutrient contents and a stable C/N ratio. The experiment was carried out from October 2024 to April 2025 arranged in a completely randomized design with four moisture levels (40, 50, 60, and 70%) applied to four biofertilizer formulations (BFs), consisting of carrier–substrate mixtures (rice husk ash, maize stems, maize leaves) at a 1: 1: 3 ratio. The formulations included: BF1 containing Cereibacter sphaeroides M-Sl-09, BF2 containing Rhodopseudomonas thermotolerans M-So-11, BF3 containing Rhodopseudomonas palustris M-So-14, and BF4 containing a mixture of the three strains, and stored for six months. Results showed that bacterial densities in the biofertilizers were highest at 60% moisture, with values of 5.93, 5.68, 5.95, and 5.85 × 10⁶ CFU g⁻¹ for BF1, BF2, BF3, and BF4, respectively. Nutrient contents of N, P, and K in the four formulations ranged 1.01–1.26%, 0.184–0.256%, and 0.751–1.07%, respectively. In addition, the C/N ratios of BF1, BF2, BF3, and BF4 were 15.0, 14.6, 14.7, and 15.4, respectively.

Keywords

Agricultural by-products biofertilizer storage potassium-solubilising bacteria 0 0);"> purple nonsulfur bacteria

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