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Crop Research

GC-MS-MS profiling and toxicity of ethyl acetate extracts of some botanicals against rice weevil, Sitophilus oryzae (L.) in stored maize 



DOI: 10.31830/2454-1761.2023.CR-913    | Article Id: CR-913 | Page : 194-201
Citation :- GC-MS-MS profiling and toxicity of ethyl acetate extracts of some botanicals against rice weevil, Sitophilus oryzae (L.) in stored maize. Crop Res. 58: 194-201
N. ANANDHABHAIRAVI, M. SHANTHI, S. VELLAIKUMAR, S. ARIVARASAN AND P. JAYAPAL anandhabhairavi03@gmail.com
Address : Department of Agricultural Entomology, School of Agriculture, VELS Institute of Science, Technology & Advanced Studies (VISTAS), Velan Nagar, Chennai-600 117 (Tamil Nadu), India
Submitted Date : 19-05-2023
Accepted Date : 28-06-2023
Online Published:

Abstract

Ethyl acetate extracts of certain botanicals were evaluated in the laboratory for their contact and oral toxicity at 5% concentration against Sitophilus oryzae L. at Natural Pesticide Laboratory, Department of Agricultural Entomology, Agricultural College and Research Institute, Madurai, Tamil Nadu during 2019-21. The result revealed that all the botanicals were effective against S. oryzae compared to the control. Considering the contact toxicity, 5% ethyl acetate extract of Mentha spicata (86.67%) performed better 72 h after treatment. Vitex negundo and Ocimum sanctum caused (83.33%) mortality 72 h after treatment concerning oral toxicity. Mentha spicata caused 93.33% mortality after 15 days of treatment, whereas Vitex negundo, Ocimum sanctum and Tagetes erecta leaf caused 90.00% mortality, which was statistically at par with Mentha spicata. GC-MS-MS study revealed the presence of carvone (38.27%) at the retention time of 7.644, Hexadecanoic acid (2.13%) at the retention time of 27.600, Isopulegol (1.41%) at the retention time of 27.290 and phytochemicals with insecticidal potential in Mentha spicata.Whereas in Ocimum sanctum, Eugenol (59.72%) was the major compound at the retention time of 9.9. Hence, it was concluded that among the tested ethyl acetate extracts, Mentha spicata at 5% was the most effective as a contact and oral toxicant against S. oryzae in stored maize, which possesses the bioactive principle of carvone and it can be developed as an eco-friendly botanical formulation.

Keywords

Botanicals carvone ethyl acetate Mentha spicata Sitophilus oryzae toxicity

References

Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18: 265-67.
Aggarwal, K. K., Khanuja, S. P. S, Ahmad, A., Santha Kumar, T. R., Gupta, V. K. and Kumar, S. (2002). Antimicrobial activity profiles of the two enantiomers of limonene and carvone isolated from the oils of Mentha spicata and Anethum sowa.  Flavour Fragr. J. 17: 59-63.
Anandhabhairavi, N., Shanthi, M., Chinniah, C., Geetha, R. and Vellaikumar, S. (2021). Evaluation of ethyl acetate extract of some botanicals against rice weevil, Sitophilus oryzae L. (Coleoptera: Curculionidae) in stored maize. Madras Agric. J. 108: 1.
Anandhabhairavi, N., Shanthi, M., Chinniah, C., Geetha, R. and Vellaikumar, S. (2022). Efficacy of hexane extracts of some plants against rice weevil, Sitophilus oryzae (L.) in stored maize. Indian J.  Entomol. 84: 368-72.
Ainane, A., Fatima, K., M'hammed, E. L., Mohammed. T., Adyl, O., Adil, L., Abdelouahed, O. and Tarik, A. (2019). Evaluation of the toxicity of the essential oils of certain mints grown in the region of Settat (Morocco): Mentha piperita, Mentha pulegium and Mentha spicata against Sitophilus granarius, Sitophilus oryzae and Sitophilus zeamais. J. Analyt. Sci.  Appl. Biotechnol. 1: 2021-10.
Aparna, V., Kalarickal, V. D., Pradeep, K. M., Ponnuraj, K., Chittalakkottu, S. and Madathilkovilakathu, H. (2012). Anti-inflammatory property of n-hexadecanoic acid: Structural evidence and kinetic assessment. Chem. Biol. Drug Des. 80: 434-39.
Awasthi, P. K. and Dixit, S. C. (2007). Chemical compositions of Ocimum sanctum Shyama and Ocimum sanctum Rama oils from the plains of northern India. J. Essent  Oil-Bear Plants 10: 292-96.
Batta, Y. A. (2004). Control of rice weevil (Sitophilus oryzae L., Coleoptera: Curculionidae) with various formulations of Metarhizium anisopliae. J. Crop Protec. 23: 103-08.
Chaubey, M. K. (2016). Insecticidal activities of Cinnamomum tamala (Lauraceae) essential oil against Sitophilus oryzae L. (Coleoptera: Curculionidae).  Int. J. Entomol. Res. 4: 91-98.
García, M., Osvaldo, J. D., Carlos, E. A., Carlos, E. T. and Marta, E. S. (2005). Toxic and repellent effects of Baccharissalicifolia essential oil on Triboliumcastaneum. Pest Manage. Sci. formerly Pestic. Sci. 61 : 612-18.
Gomez, K. A. and Gomez, A. A. (1984). Statistical Procedures for Agricultural Research. John Wiley & Sons.
Haouel-Hamdi,  S., Soltani, A., Jmal, R., Messaoud,  C., Zaouali, Y., Boushih, E. and Jemaa, J. M. B. (2021). Use of binary mixtures of three Mentha essential oils for the control of rice weevil, Sitophilus oryzae (Curculionidae). Int. J. Trop. Insect Sci. 41: 1333.
Jain, P. K., Anjali, S., Preeti, J. and Jeetendra, B. (2016).  Phytochemical analysis of Mentha spicata plant extract using UV-VIS, FTIR and GC/MS technique.  J. Chem. Pharm. Res. 8: 1-6.
Jayakumar, M., Arivoli, S., Rajasingh, R. and Tennyson, S. (2017). Repellent activity and fumigant toxicity of a few plant oils against the adult rice weevil, Sitophilus oryzae Linnaeus 1763 (Coleoptera: Curculionidae).  J. Entomol. Zool. Stud. 5: 324-35.
Kim, D. and Ahn, Young‐Joon (2001). Contact and fumigant activities of constituents of Foeniculum vulgare fruit against three coleopteran stored-product insects. Pest Manage. Sci. 57: 301-06.
Mahendra Mallah, Rekha Sapkota and Bishnu Prasad Kandel (2018). Efficacy evaluation of common botanicals to manage maize weevil (Sitophilus zeamais M.) in laboratory condition. Farm. Manage. 3: 142-45.
Melo, C. R., Arie, F. B., Bruna, M. S. O., Santos, A. C. C, Cristaldo, P. F., Araújo, A. P. A. and  Leandro Bacci (2021). Formicidal activity of essential oils of Myrcia lundiana chemotypes on Acromyrmex balzani. Crop Protec. 139: 105343.
Mohale, S., Allotey, J. and Siame, B. A. (2010). Control of Tribolium confusum J. du Val by diatomaceous earth (Protect It™) on stored groundnut (Arachis hypogaea) and Aspergillus flavus link spore dispersal.  African J. Food, Agric. Nutr. Dev. 10: 2678-94.
Park, C., Soon, K. and Ahn, Y. (2003). Insecticidal activity of asarones identified in Acorus gramineus rhizome against three coleopteran stored-product insects. J. Stored Prod. Res. 39: 333-42.
Pascual-Villalobos, M. J., Ballesta-Acosta, M. C. and Soler, A. (2004). Toxicidad y repelencia de aceitesesenciales en plagas de almacéndelarroz. Bol. San. Veg. Plagas 30: 279-86.
Rahman,  M. A., Taleb, M. A. and Biswas, M. M. (2003). Evaluation of botanical product as grain protectant against grain weevil, Sitophilus granarious (L.) on wheat. Asian J. Pl. Sci. 2: 501-04.
Rahuman, A. A., Geetha, G. B., Saleem, G.,  Arumugam, S. and  Himalayan, B. (2000). Effect of Feronia limonia on mosquito larvae. Fitoterapia 71: 553-55.
Rani, P. U. (2012). Fumigant and contact toxic potential of essential oils from plant extracts against stored product pests. J. Biopest. 5: 120.
Rani, S. S., Justin, C. G. L. and Sheeba, S. (2019). Evaluation of plant extracts against rice weevil, Sitophilus oryzae (Coleoptera: Curculionidae) in stored sorghum seeds. Pharma Innov. J. 8: 154-59.
Shahbazi, Y. (2015). Chemical composition and in vitro antibacterial activity of Mentha spicata essential oil against common food-borne pathogenic bacteria. J. Pathogens Article ID 916305.
Thomas, K. J., Selvanayagam, M., Raja, N. and  Ignacimuthu, S. (2002). Plant products in controlling rice weevil, Sitophilus oryzae. Int. Multidiscip. Res. J. 61: 269-74.
Valcarcel, F., Olmeda, A. S., Gonzalez, M. G., Andrés, M., Rocha,  J. N. and Azucena, G. C. (2021). Acaricidal and insect antifeedant effects of essential oils from selected aromatic plants and their main components. Front. Agron. 3: 662802.

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