Loading...

Crop Research

Mutagenic effect of gamma rays and EMS on agronomical traits of niger [Guizotia abyssinica (L. f.) Cass.]

DOI: 10.31830/2454-1761.2023.CR-897    | Article Id: CR-897 | Page : 83-89
Citation :- Mutagenic effect of gamma rays and EMS on agronomical traits of niger [Guizotia abyssinica (L. f.) Cass.]. Crop Res. 58: 83-89
SURUCHI V. VYAWAHARE, RAVIKIRAN PAGARE AND PRACHI A. SAOJI suruchi.umalkar@gmail.com
Address : Department of Botany, School of Science, Sandip University, Mahiravani, Trimbak Road, Nashik-422 213 (Maharashtra), India
Submitted Date : 9-12-2022
Accepted Date : 23-01-2023
Online Published:

Abstract

This study was conducted to augment genetic variability in niger plant [Guizotia abyssinica (L. f.) Cass.] using gamma rays and ethyl methane sulphonate (EMS) Niger (G. abyssinica) seeds of cultivar N-71 were exposed to various doses of gamma rays which ranged from 24 to 28 KR and different concentrations of EMS which ranged from 0.2 to 0.4% and in combination. Morphogenic and agronomic traits in the M1 and M2 generations that analyzed included: percentage of germination and survival, plant height, number of branches, capitulum size, number of ray florets per capitulum, number of disc florets per capitulum, head size, number of capitula per plant, number of seeds per capitulum, seed yield per plant and 1000-seed weight. The results of the present study showed that the three gamma irradiation doses used (24, 26 and 28 KR) had decreasing effect in most of the agronomic and morphological traits evaluated in M1. However, combination treatments like 24 KR+0.2% EMS and 26KR+0.4% EMS showed a noteworthy increase of grain yield and yield related components. In general, for the three irradiation doses in M1 population, there were no significant changes observed in morphological traits, however, in progenies of collective mutagenic treated seeds some potential high-yielding mutants were identified.

Keywords

Agronomical traits ethyl methane sulphonate gamma-ray generation niger

References

Ariharasutharsan, G., Parameswari, C., Sandhiya, V., Murugan, E. and Vanniarajan, C. (2022). Effect of gamma ray and gamma ray + EMS combination treatment on M1 and M2 generations of white seeded sesame (Sesamum indicum). Res. Crop. 23: 92-99.
Arisha, M. H. (2014). Kill curve analysis and the response of first-generation Capsicum annuum L. B12 cultivar to ethyl methane sulfonate. Genet. Mol. Res. 13: 10049-61.
Awan, M. A., Konzak, C. F., Rutger, J. N. and Nilan, R. A. (1980). Mutagenic effects of sodium azide in rice. Crop Sci. 20: 663-68.
Bhatia, C. R. (1999). Release of gene silencing in transgenics–A new role for induced mutations. Mutat. Breed. Newslett. 44: 3-5.
Botticella, E., Sestili, F., Hernandez-Lopez, A., Phillips, A. and Lafiandra, D. (2011). Higher solution melting analysis for the detection of EMS-induced mutations in wheatsheaf genes. BMC Plant Biol. 11: 156.
Datta, P. C., Helmersson, S., Kebedu, E. and Alemaw, G. (1994). Variation in lipid composition of niger seed [Guizotia abyssinica (L. f.) Cass.] samples collected from different regions in Ethiopia. J. Amer. Oil Chem. Soc. 71: 839-43.
Hiremath, S. C. and Murthy, H. N. (1988). Domestication of niger (Guizotia abyssinica). Euphytica 37: 225-28.
Kishor, H., Prabhuling, G., Ambika, D. S. and Abhijith, Y. C. (2017). Chemical induced mutations for development of resistance in banana cv. Nanjanagudu Rasabale. Crop Res. 52: 234-39.
Maluszynski, M. (1990). Gene manipulation in plant improvement, Vol. 2. Gustafsson, J. P. (ed.). Plenum Press, New York.
Misra, R. C., Mohapatra, B. D. and Panda, B. S. (2001). High yielding mutants of black gram variety PH-25. Mutat. Breed. Newslett. 45: 39.
Page, D. R. and Grossniklaus, U. (2002). The art and design of genetic screens: Arabidopsis thaliana. Nat. Rev.Genet. 3: 124-36.
Ramesh, B., Prasad, B. K. and Singh, V. P. (2001). Semi-dwarf, high yielding and high protein mutants in barley. Mutat. Breed. Newslett. 45: 26-27.
Riley, K. W. and Belayneh, H. (1989). Niger. In: Oil Crops of the World. McGraw-Hill Publishing Company, New York. Pp. 394-403.
Sarwar, M. F., Sarwar, M. H., Sarwar, M., Qadri, N. A. and Moghal, S. (2013). The role of oilseeds nutrition in human health: A critical review. J. Cereals Oilseeds 4: 97-100.
Sakin, M. A. and Yildirim, A. (2004). Induced mutations for yield and its components in durum wheat (Triticum durum Defs.). Food Agric. Environ. 2: 285-90.
Singh, S., Richharia, A. K. and Joshi A. K. (1998). An assessment of gamma ray induced mutations in rice (Oryza sativa L.). Indian J. Genet. 58: 455-63.
Venkatachalam, P., Geetha, N. and Jayabalan, N. (1999). Twelve new groundnut (Arachis hypogaea L.) mutated germplasm registered in ICRISAT gene bank. Mutat. Breed. Newslett. 44: 15-17.
Wu, Ziming (2007). A chlorophyll-deficient rice mutant with impaired chlorophyllide esterification in chlorophyll biosynthesis. Plant Physiol. 145: 29-40.
 
 

Global Footprints