Biotin a vitamin of the B-complex vitamin family well known to play a major role to improve skin, hair growth, and nails. A nutrition bar with rich biotin content is prepared. Based on this, a study was conducted using various food materials wherein three samples were prepared with different compositions and proportion. Sensory evaluation was done by the sensory panelist and scores and one sample is selected. The main sample was prepared with the selected sample combination and proportion. Then the main sample was packed in three different packaging materials and stored in two different temperature conditions. After the 15th and 30th days of packing, microbial analysis was done. By calculating the microbial analysis, sample packed in aluminum foil has a less bacterial forming colony in both room and refrigerant temperature, when compared to sample packed in low-density polyethylene and plastic bottle. The sample was found edible even after 30 days of packing as jaggery was used as a preservative and cardamom as flavoring agent having anti-fungal and anti-bacterial properties.