Soil is the major component of earth’s ecosystem which comprises of, minerals, gases, organic matter and large numbers of living organism (macro and microorganisms). The microorganism’s population is extensively responsible in regulating and developing plant growth. The study was conducted during 2017 to 2019 at Department of Botany, Institute of Basic Sciences, Bundelkhand University, Jhansi attempted to explore the diversity among mycoflora in the soil samples collected from different villages of Banda and Mahoba districts of Uttar Pradesh, India. A total of twenty-eight fungal species belonging to 15 genera were isolated from collected soil samples from chickpea fields. Mycoflora were isolated by using serial soil dilution technique on potato dextrose agar media (PDA). Five species of Aspergillus (Aspergillus candidus, Aspergillus flavus, Aspergillus fumigates, Aspergillus niger and Aspergillus oryzae), five species of Trichoderma (Trichoderma harzianum, Trichoderma koningii, Trichoderma pseudokoningii, Trichoderma variance and Trichoderma viride), three species of Fusarium (Fusarium oxysporum, Fusarium oxysporum f. sp. ciceris and Fusarium solani) and two species of Penicillium (Penicillium citrium and Penicillium chrysogenum) were most dominant among all the genera isolated.