Throughout the world, shoot and fruit borer, Leucinodes orbonalis (Guenee) is a constant pest on brinjal. Experiments were carried out to investigate the acute toxicity of spinetoram 12 SC against larval stage of L. orbonalis in the laboratory and to evaluate the effectiveness in the field in two seasons from 2013 to 2014 in Madurai district. Toxicity test on a laboratory strain of the 4th instar larva of L. orbonalis was carried out at different concentrations after 24, 48 and 72 h after treatment. LC50’s of spinetoram in 4th instar larvae after 24, 48 and 72 h were 3.84, 1.92 and 1.41 ppm, respectively, and LC95’s of spinetoram in 4th instar larvae after 24, 48 and 72 h were 15.97, 7.21 and 6.68 ppm, respectively. In field experiments, spinetoram 12 SC was significantly effective at 45 and 54 g a. i./ha when sprayed thrice at 15 days interval and minimized the incidence of shoot and fruit borer and increased the fruit yield.