Mixed liquor suspended solids (MLSS) is the concentration of suspended solids in an aeration tank during the activated sludge process, which occurs during the treatment of waste water. Mixed liquor is a combination of raw or unsettled wastewater or pre-settled wastewater and activated sludge within an aeration tank. MLSS consisted mostly of microorganisms and non-biodegradable suspended matter. Unlike previous research, this study aimed at evaluating the possibility of using primary wastewater from aeration tank containing high suspended solids (MLSS) from paper factory as the whole nutrients source for beneficial microbial growth. The beneficial microbes were then mixed with suitable carrier and used for plant growth promotion. The results of the above study revealed that the MLSS based biofertilizer was equally efficient with conventional biofertilizers in promoting the germination and growth of tomato.