An experiment was conducted to study the effect of liquid bio-nutrients along with inorganic fertilizers on yield, quality and uptake of nutrients by summer cowpea at Agronomy Instructional Farm, Chimanbhai Patel College of Agriculture, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar during summer 2012. The results of experiment showed that foliar spray of Panchgavya @ 6% at flowering+soil application of Jivamrut @ 500 l/ha at 20 DAS along with 75% RDF proved superior and registered significantly higher seed yield, stover yield, root nodules per plant as well as quality attributes viz., protein content and chlorophyll index of cowpea leaves. It also improved N and P content in seeds and stover and total uptake of N and P by summer cowpea.