In this research during 2011–12, Colletotrichum graminicola was isolated from Echinochloa spp. and studied as a biological agent for control. At first, reactions of five rice cultivars to C. graminicola were evaluated. Then, C. graminicolawas inoculated on Echinochloa spp. Results indicated that the disease rating caused by this fungus in the weed was more than that in the studied rice cultivars. Also, the fungus had a significant effect on the height, fresh weight and dry weight of Echinochloa spp. and reduced them. So, C. graminicola is not recommended as a probable mycoherbicide for biological control of Echinochloa spp. in paddy fields.