Tuberlets of nine TPS populations produced from nursery bed and transplanted seedlings in the field methods were evaluated for the study of seed health status during 2010–11 and 2011–12 rabi crop. The leaf spot and viral diseases were recorded 1–7 and 1–9%, respectively, on the foliage of crop produced from seedling transplanted tuberlets. The crop from nursery bed produced tuberlets was more vigorous and almost free from diseases which could be used as healthy planting material in the eastern plains. Populations PS 6/47-1 x D-150 and PS 6/75-6 x D-150 yielded significantly higher than control 92PT-27 in both the methods among all the populations.