A field experiment was conducted during rainy seasons of 2010 and 2011 to study the effect of sulphur and biofertilizers on nutrient contents and uptake of blackgram. The nutrient contents in grain and straw viz., N, P, K and S deviated significantly due to sulphur levels and biofertilizers as well as their interactions. The highest sulphur level (60 kg/ha) and dual biofertilizers (Rhizobium+PSB) resulted in almost significantly higher N, P, K and S contents and their uptake of blackgram. The highest uptake of nutrients by blackgram producing a total biomass up to 31.36 q/ha with highest S level was 99.56 kg N, 11.70 kg P, 52.07 kg K and 5.41 kg S/ha. Similarly, under dual biofertilizers, the corresponding uptake values were 101.61 kg N, 10.62 kg P, 53.20 kg K and 5.57 kg S/ha. The findings suggest that due to heavy withdrawal of nutrients by blackgram cv. JU-2, the succeeding crop must be nourished properly based on nutrients status of the soil.