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Cold plasma seed priming enhances salinity tolerance in sweet pepper (Capsicum annuum L.)


Citation :- Cold plasma seed priming enhances salinity tolerance in sweet pepper (Capsicum annuum L.). Res. Crop. 27: 142-149
SURA M. ABDUL MAJEED AND MUSHTAK F. KAROMI KISKO mushtakkisko@csw.uobaghdad.edu.iq
Address : Department of Biology, College of Science, University of Baghdad, Baghdad, Iraq
Submitted Date : 29-01-2026
Accepted Date : 6-02-2026

Abstract

Salinity is a major environmental constraint that limits sweet pepper productivity by causing ionic imbalance, oxidative stress, and metabolic disruption. This study assessed non-thermal dielectric barrier discharge (DBD) cold plasma as a seed-priming technique to enhance salinity tolerance in vitro conditions and at the pot stage. Seeds were exposed to cold plasma for 60 and 180 seconds and germinated under different NaCl concentrations. Growth traits, physiological responses, antioxidant enzyme activities, and the expression of the stress-related gene CaPOD were investigated. The results revealed that plasma treatment, especially at 180 seconds, significantly improved germination percentage (91.67%) compared with untreated controls (75%) and enhanced plant height, leaf area, and root dry weight. Under salinity stress, plasma-treated plants exhibited increased antioxidant capacity, as indicated by higher catalase (0.874 U/mL) and superoxide dismutase (148.28 U/mL) activities, along with greater proline accumulation. Gene expression analysis revealed significant upregulation of CaPOD under salinity conditions, with further enhancement following plasma priming; CaPOD showed the strongest response. Overall, DBD cold plasma seed priming represents an effective and eco-friendly approach to improving salinity tolerance in sweet pepper by enhancing antioxidant defence mechanisms and osmoprotection.

Keywords

Antioxidant enzymes cold plasma (DBD) gene expression salinity stress sweet pepper


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