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Genetic diversity of Phalaenopsis spp. orchids based on ISSR (Inter Simple Sequence Repeat) markers

Citation :- Genetic diversity of Phalaenopsis spp. orchids based on ISSR (Inter Simple Sequence Repeat) markers. Res. Crop. 25: 648-654
SRI HARTATI, SAMANHUDI, ACHMAD FATCHUL AZIEZ AND CHIKA ELDITA tatik_oc@yahoo.com
Address : Department of Agrotechnology, Faculty of Agriculture, Universitas Sebelas Maret, Surakarta, Central Java, Indonesia
Submitted Date : 16-09-2024
Accepted Date : 15-10-2024

Abstract

Genus Phalaenopsis is a popular orchid genus known for its large flowers and varied colors. In the wild, the presence of Phalaenopsis is rare, making propagation a necessary effort for conservation. However, research on the diversity of orchids of the genus Phalaenopsis spp has not been widely carried out. This study aims to determine the genetic diversity of orchids of the genus Phalaenopsis spp (P. deliciosa, P. venosa, P. amboinensis, P. amabilis, P bellina, P. violacea) using ISSR markers. The 5 primers used were, UBC-810, UBC-834, UBC-814, UBC-826, and UBC-843. The genetic diversity facts may be used as the basis for plant breeding method. The DNA samples were isolated and then the concentration and purity were measured. DNA amplification of the samples used the PCR (Polymerase Chain Reaction) with 5 ISSR primers. The NTSYS-pc program (Numeral Taxonomic System) version 2.02 was used to evaluate the scoring data, and the results showed up as cluster dendrograms. The results showed that 6 samples of Phalaenopsis spp were divided into 2 clusters, with similarity index values in the range of 30%-81% and genetic diversity in the range of 19%-70%.

Keywords

Dendrogram genetic diversity ISSR markers orchids Phalaenopsis similarity index

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