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Research on Crops

Research on in vitro propagation techniques of vine-tea plant (Ampelopsis cantoniensis (Hook. & Arn.) Planch)

DOI: 10.31830/2348-7542.2024.ROC-1119    | Article Id: ROC-1119 | Page : 655-661
Citation :- Research on in vitro propagation techniques of vine-tea plant (Ampelopsis cantoniensis (Hook. & Arn.) Planch). Res. Crop. 25: 655-661
LA THI THU HANG AND NGUYEN HO LAM nholam@hueuni.edu.vn
Address : University of Agriculture and Forestry, Hue University, Vietnam
Submitted Date : 21-08-2024
Accepted Date : 29-10-2024
Online Published:

Abstract

Vine-tea plant has the scientific name Ampelopsis cantoniensis (Hook. & Arn.) Planch and is a valuable herb plant in Vietnam. This study aimed to find a method to sterilize samples and nutrient medium components in each stage of shoots regeneration, rapid shoots multiplication, complete plant creation and in vitro seedling cultivation in the nursery stage of in vitro propagation techniques of vine-tea plant. Four experiments in research room and one experiment in nursery were conducted from January to December 2023 at Hue University of Agriculture and Forestry, Vietnam. Criterias of infected, alived, clean and dead samples, rate of shoot regeneration and number of shoots/samples were measured. Research results showed that the best medium to regenerate shoots from stem section carrying dormant eyes is MS + 1.5mg BAP/l + 6.5g agar/l + 30g sucrose/l.  The best medium to quickly multiply shoots is MS + 1.5 mg BAP/l + 0.2 mg IBA/l + 6.5g agar/l + 30g sucrose/l. The best complete plant-creating medium is MS + 0.5 mg α-NAA/l + 6.5g agar/l + 30g sucrose/l. In the condition that the nursery has a rain cover roof, a black net reduced the light intensity by 70% and the tissue culture nursery substrate of humus soil under the forest canopy + coconut fiber powder + smoked rice husk (1:1:1) gave the best results with survival rate reached 76.67%.

Keywords

In vitro culture propagation substrate vine-tea plant

References

Anburaj, J., Ravider Singh, C., Kuberan, T., Sundaravadivelan, C. and Kumar, P. (2011). Effects of plant growth regulators on callus induction from leaf explants of Cleome viscose. Res. J. Pharm. Biol. Chem. Sci. 2: 576-83.
Danso, K. E., Azu, E., Elegba, W., Asumeng, A., Amoatey, H. M. and Klu, G. Y. P. (2011). Effective décontamination and subséquent plantlet regeneration of sugarcane (Sacchrum officinarum L.) in vitro. Int. J. Integra. Biol. 11:  90-96.
Jafari, N., Othman, R, Y. and Khalid, N. (2011). Effect of benzylaminopurine (BAP) pulsing on in vitro shoot multiplication of Musa acuminata (banana) cv. Berangan. Afr. J. Biotechnol. 10: 2446-50. doi:10.5897/AJB10.1149.
Lam, N. H, Dong, T. P., Tich, P. V., Hoang, L. and Tuan, N. T. (2019). The Current production of Ampelopsis cantoniensis (Hook. & Arn.) Planch (vine tea) in the Tu Commune, Dong Giang District, Quang Nam Province. Vietnam J. Agric. Sci. 17: 443-53.
Loc, N. H. (2011). Plant cell and tissue culture - concepts and applications. Hue University Publishing House. doi:10.1007/978-3-030-49098-0.
Long, N. T., Hang, L. T. T. T., Ha, T. T. T., Thuy, D. T. and Cuong, L. N. (2021). Research on creating starting material sources for in vitro propagation of Petunia hybrida L. flowers. Vietnam Agricultural Science and Technology Magazine 63: 53-56.
Nguyet, L. T. N., Nga, N. T. T. and Phuong Nhi, P. T. P. (2019). Procedure for in vitro propagation of Acacia auriculiformis A. Cunn. Ex benth line CLT43. Hue University Science Magazine. No. 3D. pp: 33-41.
Tam, H. T., Nhung, T. T., Phuong, H. T. N., Cuc, N. T. K. and Do, L. T. (2020). Improve in vitro propagation coefficient and survey the adaptation process outside the nursery of laba banana (Musa sp.). National Biotechnology Conference. pp: 903-08.
Thanh, H. T. T., Thuy, N. T., Nhip, N. T., Thu, D. N., Son, N. T. and Truong, N. X. (2021). Research on rapid in vitro multiplication of vine-tea plants (Ampelopsis cantoniensis Hook. & Arn). National Biotechnology Conference 2021. Thai Nguyen University Press: pp: 702-09.
The Plant List (2024). Ampelopsis cantoniensis (Hook. & Arn.) Planch. http://www.theplantlist.org/tpl1.1/record/kew-2634478. Accessed date: July 20th, 2024.
Thu, N. T. X., Quang, V. V., Long, D. D. and Thuy, T. T. T. (2020). Structure and biological activity of five flavonoids from Ampelopsis cantoniensis. Vietnam J. Sci. Technol. 58(6A): 181-88. doi:10.15625/2525-2518/58/6A/15513.

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