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Research on Crops

Molecular characterization of Acrobeloides nanus (Nematoda: Cephalobidae) using 28S rDNA from South Africa


DOI: 10.31830/2348-7542.2024.ROC-1091    | Article Id: ROC-1091 | Page : 531-534
Citation :- Molecular characterization of Acrobeloides nanus (Nematoda: Cephalobidae) using 28S rDNA from South Africa. Res. Crop. 25: 531-534
MEHRNOUSH AMINISARTESHNIZI Mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 5-06-2024
Accepted Date : 7-08-2024
Online Published:

Abstract

Acrobeloides nanus, belonging to the family Cephalobidae (Cephalobomorpha), are bacterivores that inhabit soil. Information on bacteria-feeding nematodes is sparse. Therefore, identifying this group of nematodes is important because they are one of the main consumers of soil bacteria. Hence, study on this group of nematodes is essential. This molecular study was conducted in 2024 at Limpopo University to identify the free-living bacterivores nematodes from South Africa's soils, using a 28S rDNA marker. The nematode was extracted using the tray method, and then its DNA was extracted using the Chelex method. The nematode was identified as A. nanus. In addition, molecular sequence data of the D2-D3 region of 28S rDNA from this species are provided as DNA barcode sequences. The Nblast analysis based on the 28S rDNA showed that South African A. nanus has a 99% similarity (KX669640) with the population of the Republic of Korea. Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as A. nanus in the same clade with highly supported (89%) bootstrap values. In conclusion, this species was properly identified using 28S rDNA. However, other rDNA markers are recommended to better understand Acrobeloides phylogeny.

Keywords

28S rDNA Acrobeloides free-living nematode phylogeny

References

Aminisarteshnizi, M. (2021). Phylogenetic position of Zeldia punctata (Nematoda: Cephalobidae) using ITS rDNA from South Africa. Res. Crop. 22: 700-03.
Djigal, D., Sy, M., Brauman, A., Diop, T. A., Mountport, D., Chotte, J. L. and Villenave, C. (2004). Interactions between Zeldia Punctata (Cephalobidae) and bacteria in the presence or absence of maize plants. Plant Soil 262: 33–44.
Jay Burr, A. H. and Baldwin, J. G. (2016). The nematode stoma: Homology of cell architecture with improved understanding by confocal microscopy of labeled cell boundaries. J. Morphol. 277: 1168-86. doi.org/10.1002/jmor.20567.
Kim, D. G., Park, B. Y. and Ryu, Y. H. (2012). Soil nematode fauna in Dokdo island of Korea. Res. Plant Dis. 18: 381-86. doi.org/10.5423/RPD.2012.18.4.381.
Kim, T., Kim, J., Bae, Y. J. and Park, J. (2016). First record of Acrobeloides nanus (Cephalobidae: Rhabditida: Nematoda) from Korea. Anim. Syst. Evol. Divers. 32: 258-65.
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35: 1547–49. doi.10.1093/molbev/msy096.
Pervez, R. (2011). Acrobeloides ishraqi sp. n. and Acrobeloides mushtaqi sp. n. (Nematoda: Rhabditida) from chickpea rhizosphere, Uttar Pradesh, India. Arch. Phytopathol.  Plant Prot. 44: 1438-46. doi.org/10.1080/03235408.2010.505363.
Rybarczyk-Mydłowska, K., Mooyman, P., van Megen, H., van den Elsen, S., Vervoort, M., Veenhuizen, P., van Doorn, J., Dees, R., Karssen, G., Bakker, J. and Helder, J. (2012). Small subunit ribosomal DNA-based phylogenetic analysis of foliar nematodes (Aphelenchoides spp.) and their quantitative detection in complex DNA backgrounds. Phytopathology 102: 1153-60. doi.org/10.1094/PHYTO-05-12-0114-R.
Schiffer, P. H., Polsky, A. L., Cole, A. G., Camps, J. I. R., Kroiher, M., Silver, D. H., Grishkevich, V., Anavy, L., Koutsovoulos, G., Hashimshony, T. and Yanai, I. (2018). The gene regulatory program of Acrobeloides nanus reveals conservation of phylum-specific expression. Proc. Natl. Acad. Sci. USA 115: 4459-64. doi.org/ 10.1073/pnas.1720817115.
Sharma, H., Bharti, L., Chaubey, A. K. and Shokoohi, E. (2024). Comparative morphometrics, molecular characterization, and dynamic population of Zeldia punctata associated with mustard crops from India. Biologia 79: 1761-75. doi.org/10.1007/s11756-024-01658-w.
Shokoohi, E., Mashela, P. W. and Machado, R. A. R. (2022). Bacterial communities associated with Zeldia punctata, a bacterivorous soil-borne nematode. Int. Microbiol. 25: 207-16. doi.org/10.1007/s10123-021-00207-8.
 

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