Loading...

Research on Crops

Comparison of different parts of tangerine (Citrus tangerina) for DNA extraction 


DOI: 10.31830/2348-7542.2024.ROC-1088    | Article Id: ROC-1088 | Page : 496-498
Citation :- Comparison of different parts of tangerine (Citrus tangerina) for DNA extraction. Res. Crop. 25: 496-498
GAVIN GELDENHUYS gavin.geldenhuys@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo (Turfloop Campus), Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 29-05-2024
Accepted Date : 6-08-2024
Online Published:

Abstract

The tangerine, a citrus fruit, is grown commercially worldwide in tropical, semitropical, and warm temperate countries, including the Mediterranean region. Tangerine cultivation and production are crucial for the economy of many countries, where genomic DNA plays a critical role in genetic diversity and molecular analysis of this tree. Therefore, this study was conducted in 2022 at Limpopo University to compare DNA extraction from three different parts of the tangerine tree: fresh leaves, stems, and fruit. The Chelex method was used to extract DNA from three samples of C. tangerine from South Africa. The quantity and purity of DNA were measured using a spectrophotometer. PCR amplification with 28S primers was used to confirm and evaluate the extracted DNA. The results from the spectrophotometer showed that the highest quantity of nucleic acid was extracted from the stem (98.18± 12 mg/mL), with a 260/280 ratio for DNA quality (1.85 ± 0.01). The fruit showed a high level of protein (5.8 ± 1.8 mg/mL), but the DNA concentration was lower. In conclusion the amplification of the samples indicated that the stem had high purity and concentration of DNA, which can be suitable for PCR amplification.

Keywords

Citrus tangerine extraction PCR spectrophotometer tangerine 

References

Abdel‑Latif, A. and G, Osman. (2017). Comparison of three genomic DNA extraction methods to obtain high DNA quality from maize. Plant Methods 13: 1-9.
Aminisarteshnizi, M. (2021). Evaluating the efficiency of Chelex 100 for DNA extraction in pomegranate (Punica granatum). Res. Crop. 23: 206-10.
Aminisarteshnizi, M. (2022). Comparison of different parts of lemon (Citrus limon) for DNA extraction. Trop. Agric. 99: 383-87.
Angel, J. E., Hernández, E. G., Herrera, N. A., Gómez, L. Y., Castro, A. P., Sepúlveda, A. M. and Ebratt, E. E. (2014). Comparison of DNA extraction methods for detection of citrus huanglongbing in Colombia. Plant. Breed. Genet. Mol. Biol. 32: 7-13.
Cheng, Y. J., Guo, W. W., Yi, H. L., Pang, X. M. and Deng, X. (2003). An efficient protocol for genomic DNA extraction from citrus species. Plant Mol. Biol. Rep. 21: 177–78.
Gonza´lez-Molina, Domı´nguez-Perles, E. R., Moreno, D. A. and Garcı´a-Viguera, C. (2010). Natural bioactive compounds of Citrus limon for food and health. J. Pharm. Biomed. Anal. 51: 327-45.
HwangBo, K., Son, S. H., Lee, J. S., Min, S. R., Ko, S. M., Liu, J. R., Choi, D. and Jeong, W. J. (2010). Rapid and simple method for DNA extraction from plant and algal species suitable for PCR amplification using a chelating resin Chelex 100. Plant Biotechnol. Rep. 4: 49–52. doi:10.1007/s11816-009-0117-4.
Schlink, K. and Reski, R. (2002). Preparing high-quality DNA from Moss (Physcomitrella patens). Plant Mol. Biol. Rep. 20: 423a-423f. doi:10.1007/BF02772133.
Turan, C., Nanni, I. M., Brunelli, A. and Collina, M. (2015). New rapid DNA extraction method with Chelex from Venturia inaequalis spores. J. Microbiol. Methods 115: 139–43.    doi:10.1016/j.mimet.2015.06.005.
Turci, M., Sardaro, M. L. S., Visioli, G., Maestri, E., Marmiroli, M. and Marmiroli, N. (2010). Evaluation of DNA extraction procedures for traceability of various tomato products. Food Control 21: 143–49.
Wang, Y. C., Chuang, Y. C., and Ku, Y. H. (2007). Quantitation of bioactive compounds in citrus fruits cultivated in Taiwan. Food Chem. 102: 1163-71.
 
 
 

Global Footprints