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Research on Crops

Phylogeny of Meloidogyne incognita (Nematoda: Heteroderidae) using mtDNA from Limpopo Province, South Africa


DOI: 10.31830/2348-7542.2024.ROC-1087    | Article Id: ROC-1087 | Page : 518-521
Citation :- Phylogeny of Meloidogyne incognita (Nematoda: Heteroderidae) using mtDNA from Limpopo Province, South Africa. Res. Crop. 25: 518-521
MEHRNOUSH AMINISARTESHNIZI Mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 29-05-2024
Accepted Date : 7-08-2024
Online Published:

Abstract

Identification of Meloidogyne species is difficult using classical techniques alone, as some species have similar characteristics. Meloidogyne incognita is a plant-parasitic nematode that is one of the four most common species worldwide and has numerous hosts, including tomato (Solanum lycopersicum L.). This nematode is regarded as the most significant contributing factor to the reduction in tomato yield. Therefore, the present investigation was conducted in 2024 at the University of Limpopo to identify M. incognita. The COI mtDNA was sequenced and amplified from female nematodes, following which the blast result showed that the South African population exhibited a high similarity (100% identity) to those populations studied in China (MH743221 and MH743220). Utilizing the Maximum Likelihood method for phylogenetic analysis, it was demonstrated that our population and the other populations of M. incognita form a robust clade (87% bootstrap values).

Keywords

COI mtDNA Meloidogyne incognita molecular markers phylogeny tomato

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