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Research on Crops

Characterization of Longidorus pisi (Nematode) from South Africa: Morphological and molecular insights utilizing 18S rDNA analysis

DOI: 10.31830/2348-7542.2024.ROC-1082    | Article Id: ROC-1082 | Page : 515-517
Citation :- Characterization of Longidorus pisi (Nematode) from South Africa: Morphological and molecular insights utilizing 18S rDNA analysis. Res. Crop. 25: 515-517
MEHRNOUSH AMINISARTESHNIZI Mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 23-04-2024
Accepted Date : 7-08-2024
Online Published:

Abstract

Longidorus is a species of nematode that can be harmful to plants. Some of these nematodes are plant pests and can also transmit plant viruses such as potato virus U and cacao necrosis virus. Accurate identification of these nematodes is imperative due to their detrimental impact on agricultural crops. This study was conducted in 2022 at Limpopo University to and determine the plant-parasitic nematodes. The nematode was found and extracted using the tray method. Then, its DNA was extracted using the Chelex method. 18S rDNA was amplified using specific primers to identify the nematode. The Nblast analysis based on the small subunit ribosomal DNA showed a 99% similarity with a population of Longidorus pisi (MK172049) from Bulgaria. Additionally, phylogenetic analysis was performed using the neighbor-joining method implemented in mega x software. The analysis indicated that the South African populations of L. pisi are similar to other L. pisi, with a 99-bootstrap value. In conclusion, there is a need to investigate the diversity of this species to assess the impact of this nematode on crop yields.

Keywords

Longidorus pisi nematode phylogeny rDNA 

References

Aminisarteshnizi, M. (2021). Molecular characters of parasitic-nematode (Longidorus pisi) from regions of South Africa. Res. Crop. 22: 394-97
Bohra, P. and Baqri, Q. H. (2005) Plant and soil nematodes from Ranthambhore National Park, Rajasthan, India. Zoos Print J. 21: doi:10.11609/JOTT.ZPJ.1340.2126.
Edward, J. C., Misra, S. L. and Singh, G. R. (1964) Longidorus pisi n.sp. (Nematoda, Dorylaimoidea) associated with the rhizosphere of Pisum sativum, from Uttar Pradesh, India. Jpn. J. Appl. Entomol. Zool.. 8: 310–12.
Koohkan, M., Shokoohi, M. and Mullin, P. (2015). Phylogenetic relationships of three families of the suborder Mononchina Kirjanova & Krall, 1969 inferred from 18S rDNA. Nematology 17: 1113-25.
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol.. 35: 1547–49.
Lazarova, S. S., Elshishka, M., Radoslavov, G., Lozanova, L., Hristov, P., Mladenov, A., Zheng, J., Fanelli, E., De-Luca, F. and Peneva, V. K. (2019). Molecular and morphological characterisation of Longidorus polyae sp. n. and L. pisi Edward, Misra & Singh, 1964 (Dorylaimida, Longidoridae) from Bulgaria. ZooKeys 830: 75–98. doi:10.3897/ zookeys.830.32188.
Shokoohi, E. (2021). First report of Bitylenchus ventrosignatus (Tobar Jimenez, 1969) Siddiqi, 1986 associated with wild grass in Botswana. J. Nematol. (Inpress). doi:10.21307/jofnem-2021-037.
Tzortzakakis, E. A., Clavero-Camacho, I., Cantalapiedra-Navarrete, C., Ralli, P., Palomares-Rius, J. E., Castillo, P. and Archidona-Yuste, A. (2021). Morphological and molecular characterisation of Longidorus pauli (Nematoda: Longidoridae), first report from Greece. J. Nematol. 53: 1-10. doi:10.21307/jofnem-2021-034.
Van den Berg, E., Marais, M. and Swart, A. (2017). Nematode morphology and classification. In: Nematology in South Africa: A view from the 21st Century. Springer, Cham. doi:10.1007/ 978-3-319-44210-5_3.
Van Megen, H., Van Den E. S., Holterman, M., Karssen, G., Mooyman, P., Bongers, T., Holovachov, O., Bakker, J. and Helder, J. (2009). A phylogenetic tree of nematodes based on about 1200 full-length small subunit ribosomal DNA sequences. Nematology 11: 927-50. doi:10.1163/156854109X456862.
Xu, Y., Ye, W., Wang, J. and Zhao, Z. (2018) Morphological and molecular characterisation of Longidorus pinus sp. n. (Nematoda: Longidoridae) from China and a key to known species of Longidorus in China. Nematology 20: 619–39. doi:10.1163/15685411-00003165.
 
 

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