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Research on Crops

Study of bacterium associated with Spinacia oleracea and nematode, Pseudomonas fragi using 16S rDNA from Limpopo Province, South Africa


DOI: 10.31830/2348-7542.2024.ROC-1072    | Article Id: ROC-1072 | Page : 509-511
Citation :- Study of bacterium associated with Spinacia oleracea and nematode, Pseudomonas fragi using 16S rDNA from Limpopo Province, South Africa. Res. Crop. 25: 509-511
MEHRNOUSH AMINISARTESHNIZI Mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 7-03-2024
Accepted Date : 7-08-2024
Online Published:

Abstract

Microorganisms known as Plant-associated Pseudomonas can thrive as parasites or saprophytes on plant surfaces and within plant tissues. They are crucial in enhancing plant growth by suppressing pathogenic microorganisms, synthesizing growth-stimulating plant hormones, and boosting plant disease resistance. At the University of Limpopo in South Africa, a molecular study was conducted in 2023 to identify a Pseudomonas bacterium in a spinach field associated with a free-living nematode, Acrobeles complexus, in Limpopo Province. The study utilized the Chelex method to extract DNA and the 16S rDNA marker to identify the bacterium as Pseudomonas fragi. Specific primers were then used to amplify 16S rDNA, which identified the bacterium, and Nblast analysis revealed a 96% similarity to a strain from India. Phylogenetic analysis placed this species with those identified as P. fragi in the same clade with highly supported (100) bootstrap values. This marker (16S rDNA) is recommended to use. But for a better understanding of P. fragi's phylogeny, the other DNA markers are recommended to identify this species.

Keywords

16S rDNA bacterium phylogeny Pseudomonas

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