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Research on Crops

Comparison of two methods for DNA extraction from Guava (Psidium guajava)



DOI: 10.31830/2348-7542.2023.ROC-964    | Article Id: ROC-964 | Page : 337-340
Citation :- Comparison of two methods for DNA extraction from Guava (Psidium guajava). Res. Crop. 24: 337-340
GAVIN GELDENHUYS gavin.geldenhuys@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences, University of Limpopo (Turfloop Campus), Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 14-04-2023
Accepted Date : 2-06-2023
Online Published:

Abstract

Psidium guajava is well known tropical tree which is abundantly grown for fruit. This fruit plant is consumed as food and folk medicine in sub-tropical areas worldwide due to its pharmacological activities. Guava finds a very important place in medical systems almost worldwide. This study was conducted in 2022 at the genetic lab, University of Limpopo to compare two different extraction methods for DNA from guava. The DNA from the guava was extracted using Chelex "method 1" (overnight incubation at 56°C) and "method 2" (Ten-minute incubation at 95°C) from the fresh leaves of the guava. The quantitative and qualitative parameters were measured using a spectrophotometer. PCR reactions with primers for 28S were used on all samples to confirm and evaluate the extracted DNA. The results showed that the highest quality of extracted DNA was in "method 2" (1.68-1.82). The quantitative and qualitative tests for PCR reaction showed that the DNA extracted using "method 2" had a better quality of DNA than "method 1". In conclusion, both methods worked, but method two showed better results.

Keywords

DNA extraction PCR spectrophotometer Psidium guajava 

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