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Research on Crops

Comparison of two methods for DNA extraction from Avocado (Persea americana)

DOI: 10.31830/2348-7542.2022.ROC-851    | Article Id: ROC-851 | Page : 870-873
Citation :- Comparison of two methods for DNA extraction from Avocado (Persea americana). Res. Crop. 23: 870-873
GAVIN GELDENHUYS gavin.geldenhuys@ul.ac.za
Address : School of Agricultural and Environmental Sciences, University of Limpopo, Turfloop Campus, Private Bag X1106, Soveng a, 0727, South Africa
Submitted Date : 23-08-2022
Accepted Date : 22-09-2022
Online Published:

Abstract

Avocado fruit contains a substantial amount of triglycerides (TGs) along with a high content of unsaturated fatty acids. It is also rich in many other bioactive phytochemicals such as carotenoids, tocopherols, phytosterols, aliphatic alcohols, and hydrocarbons. This study was conducted during 2021 at the genetic lab, University of Limpopo, South Africa to compare two different extraction DNA methods from avocado. The DNA from the avocado was extracted using Chelex "method 1" (overnight incubation at 56°C) and "method 2" (Ten-minute incubation at 95°C) from the fresh leaves of the avocado. The quantitative and qualitative parameters were measured using a spectrophotometer. PCR reaction with primers for 28S were used on all samples to confirm and evaluate the extracted DNA. The results showed that the highest quality of extracted DNA was in "method 2" (1.54-1.68). The quantitative and qualitative tests for PCR reaction showed that the DNA extracted using "method 2" had better quality of DNA than "method 1". In conclusion, both methods worked, but method two showed better results.
 

Keywords

DNA extraction Persea americana spectrophotometer PCR 28S

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