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Research on Crops

Molecular characters of Enterobcater using 16S rDNA isolated from tomato, Limpopo Province, South Africa

DOI: 10.31830/2348-7542.2022.ROC-849    | Article Id: ROC-849 | Page : 858-861
Citation :- Molecular characters of Enterobcater using 16S rDNA isolated from tomato, Limpopo Province, South Africa. Res. Crop. 23: 858-861
MEHRNOUSH AMINISARTESHNIZI mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences,, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 23-08-2022
Accepted Date : 22-09-2022
Online Published:

Abstract

Enterobacter species are gram negative, motile, non-spore forming, rod shaped, facultatively anaerobic bacteria of the Enterobacteriaceae family. It is also described as non-spore-forming, flagella-containing, urease positive, and lactose fermenting. Enterobacter includes several species which can be a risky pathogenic bacteria to the vegetables, including tomato, and therefore have an impact on the fresh production of tomato. This study was conducted to isolate and identify Enterobacter from a tomato field, in Limpopo Province, South Africa. Therefore, this molecular study was conducted in 2022 at the Limpopo University to identify the bacterium associated with tomato filed from South Africa's soils using 16S rDNA marker. The DNA was extracted using the chelex method. The bacterium was identified as Enterobacter. The Nblast analysis showed South African Enterobacter has 97% similarity with a population from Korea (KC355340). Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as Enterobacter in the same clade with highly supported (100) bootstrap values. In conclusion, this species is identified using 16S rDNA properly. However, using other DNA markers for a better understanding of Enterobacter phylogeny is recommended.
 

Keywords

Bacterium Enterobacter phylogeny South Africa tomato 16S rDNA

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