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Research on Crops

Phylogenetic position of Scutellonema brachyurus (Nematoda: Hoplolaimidae) associated with tomato using 28S rDNA from South Africa



DOI: 10.31830/2348-7542.2022.ROC-848    | Article Id: ROC-848 | Page : 855-857
Citation :- Phylogenetic position of Scutellonema brachyurus (Nematoda: Hoplolaimidae) associated with tomato using 28S rDNA from South Africa. Res. Crop. 23: 855-857
MEHRNOUSH AMINISARTESHNIZI mehrnoush.aminisarteshnizi@ul.ac.za
Address : Aquaculture Research Unit, School of Agricultural and Environmental Sciences,, University of Limpopo, Private Bag X1106, Sovenga, 0727, South Africa
Submitted Date : 23-08-2022
Accepted Date : 22-09-2022
Online Published:

Abstract

Plant parasitic nematodes are the main nematodes that live in the soil causing yield loss in various crops. Scutellonema species is ectoparasite and therefore can feed on the roots of tomatoes resulting in less production. This species must be identified correctly because it is a plant-parasitic and the tomato is one of the most important vegetable crops in Limpopo Province in South Africa. Therefore, this molecular study was conducted in 2022 at Limpopo University to identify the nematode from South Africa's soils using 28S rDNA marker. The recovered nematode was extracted using the tray method, and then its DNA was extracted using the chelex method. The nematode was identified as Scutellonema brachyurus. Afterwards, 28S rDNA was amplified using specific primers to identify the nematode at the molecular level. The Nblast analysis based on the large subunit ribosomal DNA showed South African S. brachuyurus had 99 % similarity (MW504476; MW504474; JX472047; JX472045) with South Africa, and USA populations, respectively. Phylogenetic analysis using maximum likelihood placed this species with those molecularly identified as S. brachyurus in the same clade with highly supported (100) bootstrap values. In conclusion, this species was identified using 28S rDNA however, the use of other rDNA markers such as ITS rDNA and mtDNA for a better understanding of Scutellonema phylogeny is recommended.
 

Keywords

Plant parasitic nematode phylogeny Scutellonema 28S rDNA

References

Abolafia, J. and Shokoohi, E. (2017). Description of Stegelletina lingulata sp. n. (Nematoda, Rhabditida, Cephalobidae) from xeric environments in Iran. Zootaxa 4358 : 462-70.
Aminisarteshnizi, M. (2021a). Evaluation of proteinase K and boiling water for DNA extraction from Helicotylenchus sp. Res. Crop. 22 : 163-66.
Aminisarteshnizi, M. (2021b). Lipid content in the juvenile, female and male of Acrobeles complexus nematode. Res. Crop. 22 : 167-70.
Aminisarteshnizi, M. (2021c). Efficacy of neem (Azadirachta indica) formulations on suppression of root-knot nematodes (Meloidogyne javanica) and growth of eggplant (Solanum melongena). Res. Crop. 22 : 158-62.
Kolombia, Y., Karssen, G., Viaene, N., Kumar, L., Joos, L., Coyne, D. and Bert, W. (2017). Morphological and molecular characterization of Scutellonema species from yam (Dioscorea spp.) and a key to the species of the genus. Nematology 19 : 751-87.
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. (2018). MEGA X: molecular evolutionary genetics analysis across computing platforms. Mol. Biol. Evol. 35 : 1547-49.
Nguyen, T., Trinh, P., Coureur, M., Singh, R., Decraemer, W. and Bert, W. (2019). First Report of Scutellonema brachyurus (Steiner, 1938). Andrassy, 1958 and Occurrence of Meloidogyne incognita (Kofoid and White, 1919) Chitwood, 1949 in Belgium. J. Nematol. 51 : 1-6.
Shokoohi, E. (2021a). Morphological and molecular characters of Scutellonema brachyurus (Steiner, 1938) Andrassy, 1958 from South Africa. J. Nematol. 53 : 1-13.
Shokoohi, E. (2021b). Morphological and molecular characterization of Boleodorus volutus Lima & Siddiqi, 1963 from South Africa with the first SEM observations of the species. Russ. J. Nematol. 29 : 101-09. 10.24412/0869-6918-2021-2-101-109
Shokoohi, E. and Abolafia, J. (2019). Soil and freshwater Rhabditid Nematodes (Nematoda, Rhabditida) from Iran: A Compendium. University of Jaen (UJA) Publishing. Pp. 226.
Shokoohi, E. and Abolafia, J. (2021). Redescription of a predatory and cannibalistic nematode, Butlerius butleri Goodey, 1929 (Rhabditida: Diplogastridae), from South Africa, including its first SEM study. Nematology 23 : 969-86.
Shokoohi, E. and Abolafia, J. (2022). Morphological and molecular characterization of Hemicycliophora poranga Monteiro and Lordello, 1978 (Nematoda: Rhabditida: Hemicycliophoridae) from Iran and South Africa. Biologia 77 : 709-20. doi.org/10.1007/s11756-021-00990-9.

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