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Research on Crops

Bio-insecticidal and seed protectant effects of four essential oils against Callosobruchus maculatus and Callosobruchus chinensis during storage 


DOI: 10.31830/2348-7542.2022.ROC-867    | Article Id: ROC-867 | Page : 676-681
Citation :- Bio-insecticidal and seed protectant effects of four essential oils against Callosobruchus maculatus and Callosobruchus chinensis during storage. Res. Crop. 23: 676-681
SABBOUR M. M. AND ABD EL-AZIZ S. E. shadia.abdelaziz77@gmail.com
Address : Pests and Plant Protection Department, Agriculture and Biological Research Institute, National Research Centre, 33rd El-Behouse St., (El-Tahrir St.) Dokki, Giza, P.O. Box 12622, Egypt
Online Published:

Abstract

         In many Integrated Pest Management (IPM) programs, many essential oils were recommended to protect plants from insect’s infestations and have few effects on non-target organisms and the environment. Four essential oils (EOs): Ocimum basilicum, Peganum harmala, Anethum graveolens and Lavandula angustifolia were evaluated against two of the most important stored product insect pests, Callosobruchus maculatuc and C. chinensis infesting cowpea seeds during storage. The tested essential oils were obtained by steam distillation methods of dried plants. Effect of Contact toxicity, insecticidal of lethal concentrations, Oviposition deterrent activity, effect on germination and weight loss of seeds of tested oils were experimented on tested insects. The highest conc. 1% achieved (93, 80 and 77%) in the case of O. basilicum, P. harmala, A. graveolens, respectively in the case of C. maculatus. While in C. chinensis 1% conc. achieved, 90, 72 and 67% in the case of O. basilicum, A. graveolens and P. harmala, respectively. The LC50 of tested oils showed that the LC50 of O. basilicum was significantly decreased 4-fold more than lavender oil and O. basilicum is the best oil against C. maculatus and C. chinensis. Cowpea seeds treated with O. basilicum at high rate (1%) showed maximum prevention in oviposition i.e., 99% followed by A. graveolens causing (63 and 55%) prevention in the oviposition of C. maculatus and C. chinensis, respectively. Least weight loss was observed in O. basilicum and A. graveolens in the case of C. maculatus (4.44±0.47 and 8.13 ± 0.32) and C. chinensis (3.14±0.33 and 6.17 ± 0.12), respectively. O. basilicum treatment achieved the highest Feeding Deterrent Index (FDI) of 92.99 and 89.88% in the case of C. chinensis and C. maculatus, respectively. Least (FDI) was recorded in case of L. angustifolia (36.72 and 32.46%) in the case of C. chinensis and C. maculatus, respectively. Our results confirmed that O. basilicum essential oil had an obvious role in suppressing the activity of the tested insects. O. basilicum essential oil can be used in an integrated pest management program as green pesticide and can be effective alternative for chemical pesticides.

Keywords

Basil chickpea beetle contact toxicity cowpea beetle dill lavender

References

Abbott, W. W. (1925). A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 18 : 265-67.
Ashamo, M. O., Ileke, K. D. and Onasile, A. I. (2021). Chemical composition and toxicity of some agro waste-derived insecticides against Angoumois grain moth, Sitotroga ceralella (Olivier) [Lepidoptera: Gelechiidae] infesting stored paddy grains. Bull. Nat. Res. Cent. 45 : 1-12.
Campolo, O., Giunti, G., Russo, A., Palmeri, V. and Zappala, L. (2018). Essential oils in stored product insect pest control. J. Food Qual. 2018 : doi.org/10.1155/2018/6906105.
Chiluwal, K., Kim, J., Do Bae, S. and Gyoo Park, C. (2017). Essential oils from selected wooden species and their major components as repellents and oviposition deterrents of Callosobruchus chinensis (L.). J. Asia-Pacific Entomol. 20 : 1447-453.
Cruz, G., Wanderley-Teixeira, V., Marques da Silva, L., Andrade Dutra, K., Arruda Guedes, C., Vargas de Oliveira, J., Ferraz Navarro, D. M. A., Caldas Araújo, B., Aguiar, A. and Teixeira, C. (2017). Chemical composition and insecticidal activity of the essential oils of Foeniculum vulgare Mill., Ocimum basilicum L., Eucalyptus staigeriana F. Muell. ex Bailey, Eucalyptus citriodora Hook and Ocimum gratissimum L. and their major components on Spodoptera frugiperda (Lepidoptera: Noctuidae). J. Essent. Oil Bear. Pl. 20 : 1360-369.
De, A., Bose, R., Kumar, A. and Mozumdar, S. (2014).  Worldwide pesticide use. In: De, A., Bose, R., Kumar, A. and Mozumdar, S. (Eds). Targeted delivery of pesticides using biodegradable polymeric nanoparticles. New Delhi: Springer. Pp. 5-6.
Finney, D. J. (1971). Probit analysis. Cambridge University Press, London.
Ilboudo, Z., Dabiré-Binso, C. L., Sankara, F., Nébié, R. C. H. and Sanon, A. (2015). Optimizing the use of essential oils to protect stored cowpeas from Callosobruchus maculatus (Coleoptera: Bruchinae) damage. Afr. Entomol. 23 : 94-100.
Islam, T., Iqbal, J., Abdullah, K. and Khan, E. A. (2017). Evaluation of some plant extracts against maize weevil, Sitophilus zeamais (Coleoptera: Curculionidae) under laboratory conditions. Pak. J. Agric. Sci. 54 : 737-41.
Kedia, A., Prakash, B., Mishra, P. K., Singh, P. and Dubey, N. K. (2015). Botanicals as eco friendly biorational alternatives of synthetic pesticides against Callosobruchus spp. (Coleoptera: Bruchidae) - a review. J. Food Sci. Technol. 52 :1239-257.
Khanahmadi, M., Pakravan, P., Azandaryani, A. H., Negahban, M. and Ghamari, E. (2017).  Fumigant toxicity of Artemisia haussknechtii essential oil and its nano-encapsulated form. J. Entomol. Zool. Studies 5 : 1776-783.
Ozliman, S., Yaldiz, G., Camlica, M. and Ozsoy, N. (2021). Chemical components of essential oils and biological activities of the aqueous extract of Anethum graveolens L. grown under inorganic and organic conditions.  Chem. Biol. Technol. Agric. 8 : 1-16.
Rodríguez-González, A., Álvarez-García, S., González-López, O., Da Silva, F. and Casquero, P. A. (2019). Insecticidal properties of Ocimum basilicum and Cymbopogon winterianus against Acanthoscelides obtectus, insect pest of the common bean (Phaseolus vulgaris, L.). Insects 10 : doi:10.3390/insects10050151.
Sabbour, M. M. and Abd-El-Aziz, S. E. (2016). Efficacy of three essential oils and their nano particles against Sitophilus granarius under laboratory and store conditions. J. Ent. Res. 40 : 229-34.
Sabbour, M. M. and Abd-El-Aziz, S. E.  (2018). The combined effect of Metarhizium anisopliae and some natural oils against Ephestia kuehniella and Ephestia cutella (Lepidoptera-Pyralidae) under laboratory and store conditions. Biosci. Res. 15 : 3480-489.     
Sabbour, M. M. and Abd-El-Aziz, S. E. (2020). Plant essential oils for the management of two serious stored pests in Egypt. J. Ent. Res. 44 : 377-84.
Sabbour, M. M. and Abd-El-Aziz, S. E. (2021). Bioefficacy of some essential oils and nano gel chitosan on two insect species of stored pea seeds. J. Ent. Res. 45 : 647-52.
Souza, V. N., de Oliveira, C. R. F., Cysneiros Matos, C. H., de Almeida, D. K. F. (2016). Fumigation toxicity of essential oils against Rhyzopertha dominica (F.) in stored maize grain. Revista Caatinga 29 : 435-40.
Steel, R. G. D. and Torrie, J. H. (1980). Principles and procedures of statistics: A biometrical approach, 2nd Ed. McGraw-Hill, New York.
Tarek, A., Afsheen, S., Hussain, M., Zia, A., Shakeel Shah, S., Afzal, S., Khan, I. and Hayat, Y. (2017). Bioefficacy of plant powders against Callosobruchus chinensis L. (Coleoptera: Bruchidae) in infested chickpea grains. Asian J. Agric.  Biol. 5 :119-25.
Tran, D. H., Nguyen, T. G. and Bui, N. H. (2022). Efficacy of the extract from pongam (Pongamia pinnata) leaves against the cassava pink mealybug (Phenacoccus manihoti Matile-Ferrero) under laboratory conditions. Res. Crop. 23 : 197-201.

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