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Germination of chamomile (Matricaria recutita) cryopreservation using PVS2 cryoprotectant

DOI: 10.31830/2348-7542.2022.063    | Article Id: 063 | Page : 466-472
Citation :- Germination of chamomile (Matricaria recutita) cryopreservation using PVS2 cryoprotectant. Res. Crop. 23: 466-472
Saltanat Tleukenova, Alibek Ramazanov, Margarita Ishmuratova, Roza Mussina, Sairan Atikeeva, Ainur Nurkenova, Sayat Zhumadilov, Gulmira Shapatova, Aiman Sarsembaeva kairidenovich_rak@mail.ru
Address : Laboratory of Biotechnology and Ecomonitoring, E. A. Buketov Karaganda University, 28 University Street 100028, Karaganda, Kazakhstan; Design, Service and Tourism department Turan University, 29 Ykylas Dukenuly Street, 010000, Nur-Sultan, Kazakhstan; Morphology and Physiology department Karaganda Medical University, 40 Gogol Street, 100000, Karaganda, Kazakhstan

Abstract

Preservation of plant world biodiversity, especially medicinal species, is one of the main tasks of modern science. Seeds are the most optimal form of genetic material storage, as samples require little space and remain viable for a long period. There are several methods for storing plant seed. One of the most inexpensive and effective methods is cryopreservation. The present study was conducted at with an objective study the seeds of Matricaria recutita for cryopreservation and viability of seeds. The seed material was frozen in liquid nitrogen (Dewar vials) using cryoprotectant PVS2. The assessment of seed viability was conducted in laboratorial conditions on Petri dishes. The results of the study showed that cryopreservation of Matricaria recutita seeds is better to carry out without the use of PVS2 cryoprotectant, since the germination of seeds was lower than the control (56%) (seed freezing without cryoprotective substances). Seeds’ sowing after cryopreservation is recommended in two versions, immediately after defrosting and also with a delay in sowing, which makes it possible to transport thawed seeds to outlying regions.

Keywords

Cryopreservation cryoprotectant Matricaria recutita seed germination

References

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