The purpose of the research was to study the effectiveness of a biological drug ”Alirin-B”, Gamair for treatment and prevention of bacterial and some fungal diseases in various crops. Studies were conducted on fields (10 ha-experimental, 10 ha-control) and in the apple orchard (100 trees in each experimental and control groups) of the Rostov region (Russian Federation) in 2017–19. The experimental sites were treated with “Alirin-B”, Gamair, while control areas not. The experimental sites treated with “Alirin-B” showed a three times lower rate of root rot infection compared with the control (P=0.001) in two months, and 25 times less in three weeks (P=0.001). Another drug, based on Bacillus subtilis, was less effective-only two times less infection in three weeks (P=0.05) and 0.3 times less in two months. The treatment with drugs of winter wheat showed an efficiency of 50–95% (P=0.001), sugar beet-45-80% (P=0.05), apple trees-87-99% (P=0.001) and strawberries-85-95% (P=0.001).