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Molecular diagnostics of Ditylenchus destructor based on the ITSrDNA from Iran and Russia Federation

DOI: 10.31830/2348-7542.2020.025    | Article Id: 025 | Page : 151-155
Citation :- Molecular diagnostics of Ditylenchus destructor based on the ITSrDNA from Iran and Russia Federation. Res. Crop. 21: 151-155
Niloufar Mahmoudi, Mikhail Pridannikov, Meisam Zargar, Yousef Naserzadeh, Liudmila Limantceva, Elena Pakina Zargar_m@rudn.ru
Address : 1Department of Agro Biotechnology, Institute of Agriculture, RUDN University, 117198, Moscow, Russia; 2All-Russian Plant Quarantine Centre (VNIIKR), Moscow, Russia; 3The Center of Parasitology, A. N. Severtsov Institute of Ecology and Evolution, RAS, Russia

Abstract

Several nematode species related to potato have been documented. Among these, the potato rot nematode, Ditylenchus destructor (Thorne) may cause important losses in potato yields. Proper identification of nematode species and isolates is important. To verify species identification, the populations of D. destructor from Iran and the Russian Federation were tested for differences using PCR with species-specific primers for the rapid and reliable result. New sequences deposited in the Genbank under accession numbers MN307126, MN307128 and MN493767 from Iran populations, and MN658597, MN658599, MN658637 and MN658638 from Russian populations. Phylogenetic studies of the ITS-rDNA region of D. destructor put it in a distinct clade.

Keywords

Ditylenchus destructor  Iran  molecular  phylogeny  potato  Russian Federation.

References

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