Abstract

Physalis angulata L. is an important herbal plant used in the food industry or in folk medicine in most countries around the world. The present study was carried out in order to determine the secondary metabolites in the samples of callus and seedlings growing in vitro of this plant using GC/MS technique. Results showed that the MS medium supplemented with the concentration of 9.04 μM 2, 4-D was the best in the growth of callus by giving the highest biomass weight (1241 mg) of callus induced from seeds. The GC/MS analysis of chloroform extract of callus and seedlings growing in vitro revealed the presence of 25 compounds in callus, 27 in seedlings and 18 in roots. Most of the compounds were diagnosed in the callus and seedling samples with pharmaceutical and economic benefits. This indicated the importance of in vitro cultures in the production of new compounds that were medically and economically effective.