Abiotic stresses assess the main impendences or barriers to the improvement of agriculture. Subsequently, the efforts to improve stress-tolerant plants are of major importance in increasing crop productivity. In recent years, plant tissue culture techniques established in vitro have appeared as cost-effective tools and a practical method for the development of stress tolerance in plants. The relatively powerful tolerance of plant cultivar to salinity was also related to the capability of plants to accumulate increased levels of proline. The adjustment of the accumulation of proline was estimated in sodium-chloride-adapted versions of a salt-sensitive and a salt-tolerant plant, respectively, following sodium chloride shock, and defined a biotechnology technique for the enhancement of salt tolerance in crops.