A field experiment was conducted during the agricultural season of 2017–18 to study the effect of foliar application of tyrosine (Tyr) at concentrations 0, 100 and 200 mg/l, sulfur fertilization (S) at levels 0, 50 and 100 kg/ha and their interactions on growth, yield and antioxidant compounds of arugula (Eruca sativa Mill.) leaves and seeds. The treatments were distributed on factorial experiment at randomized completely block design in three replicates. Means were compared according to the Least Significant Difference test (LSD) at probability level of 0.05. Results showed that a single effect of either Tyr or S had significant impact (P<0.05) when increased concentrations in all parameters, while treat Tyr200 × S100 was given high values in antioxidant compounds including ascorbic acid, tocopherol, β-carotene, glucosinolate and glutathione to be 85.31, 6.28, 7.33, 40.22 and 12.06 mg/100 g FW in leaves and 66.51, 10.10, 4.27, 56.54 and 1.05 mg/100 g FW in seeds, respectively.