The development of cotton hybrids which can offer the great yields and quality fibre is the current research in cotton breeding. For the first time, here we investigated the mean performance and ratooning ability of 30 promising interspecific cotton hybrids. Based on mean performance, the evaluated hybrids varied significantly (P≤0.05) in first crop and ratoon crop and differed significantly in their ratooning ability for all the studied traits. The current study demonstrated that, among the tested hybrids, TCH 1716 x SUVIN showed highest ratooning ability for the seed cotton yield per plant and TCH 1716 x TCB 209 exhibited highest ratooning ability for 2.5% span length.