The experiment was conducted in private field at AL-Azizea district (70 KM) North Wasit city From September 26 to November 29 in 2017 to investigate the positive effect of ascorbic acid, amino acid (equilibrium) and nitrogen nutrition each other in single way or combination in plant height, stem diameter, total chlorophyll, total acidity, vitamin C and average of potassium and calcium in leaves of Solanum lycopersicum L. Three levels have been applied which were ascorbic acid (0, 30 and 60) mg/l, amino acid three levels (0, 1.5 and 2.5 ml/l) and nitrogen three levels (0, 0.50 and 1 g/l). Results indicated that plants treated with ascorbic acid increased all studied traits compared with control treatment. Treatment plants with amino acid in single way produced a significant increase in plant height, total chlorophyll, total acidity, vitamin C and containing leaves from potassium, while decreased stem diameter increased concentration significantly compared with control treatment. Treatment of nitrogen nutrition gave a significant effect and the highest values in the experiment. Generally, some plant qualities (plant height, stem diameter, total chlorophyll and total acidity, vitamin C, potassium and calcium) increased significantly with the rise of a two or three combination (ascorbic acid+amino acid+nitrogen nutrition) levels. Treatments are designed as a factorial experiment (3 × 3 × 3 × 3) in completely randomized design with three replicates. The purpose was to study the positive effect of ascorbic acid, amino acid (equilibrium) and nitrogen nutrition in vegetative properties and chemical content of Solanum lycopersicum L.