The purpose of this study was to examine whether the pathogenic microorganisms were detected in water and soil used as the media. After ‘Hedera helix’ and ‘Chamaedorea elegans’ which are the most used for horticultural activities were cultured in three media such as horticultural bed soil, cultural soil and tap water for 63 days, the presence of pathogenic microorganisms was examined. Pathogenic microorganisms was analyzed such as Escherichia coli O157: H7 (E. coli O157: H7) and Salmonella spp., and the method for analysis was carried out in accordance with the quality inspection on fertilizer by Rural Development Adminstration (RDA). The results of the study, pathogenic Escherichia coli O157: H7 and Salmonella spp. were not detected in the soil and water when the plants were cultivated in horticultural bed soil, cultural soil and tap water within the same conditions as the hospital environment.